Une publication de l'EA fait la couverture de Stem Cells and Development / Actualités / EA2496 / Recherche - EA2496

Une publication de l'EA fait la couverture de Stem Cells and Development

Les travaux de Soledad Acuna-Mendoza, dirigés par le Pr Anne Poliard viennent d'être publiés dans Stem Cells and Development et font l'object de la couverture du numéro 23, volume 26, 2017.

Capture d’écran 2017-12-08 à 17.18.14

Stem Cells Dev. 2017 Dec 1;26(23):1682-1694. doi: 10.1089/scd.2017.0115. Epub 2017 Nov 3.

A New Wnt1-CRE TomatoRosa Embryonic Stem Cell Line: A Tool for Studying Neural Crest Cell Integration Capacity.

Acuna-Mendoza S1,2, Martin S3, Kuchler-Bopp S4,5, Ribes S1, Thalgott J6, Chaussain C1,7, Creuzet S8, Lesot H4,5, Lebrin F3,6, Poliard A1.

Abstract

Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies, but further knowledge on their in vivo behavior is required before clinical transfer may be envisioned. We here describe the isolation and characterization of a new mouse embryonic stem (ES) line derived from Wnt1-CRE-R26 RosaTomatoTdv blastocyst and show that it displays the characteristics of typical ES cells. Further, these cells can be efficiently directed toward an NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence. As native NC progenitors, they are capable of differentiating toward typical derivative phenotypes and interacting with embryonic tissues to participate in the formation of neo-structures. Their specific fluorescence allows purification and tracking in vivo. This cellular tool should facilitate a better understanding of the mechanisms driving NC fate specification and help identify the key interactions developed within a tissue after in vivo implantation. Altogether, this novel model may provide important knowledge to optimize NC stem cell graft conditions, which are required for efficient tissue repair.