Valerie TALY

Valérie TALY (Abécassis)

valerie.taly<at>parisdescartes.fr

42 years old

Married, 2 Children

 

BIOGRAPHY

After graduation as a Molecular Toxicologist, Dr Valerie Taly (Abecassis) performed her PhD research at the Center of Molecular Genetics (CNRS, Gif-sur-Yvette, FR) on applications of combinatorial methods (generally used for proteins directed evolution) to the study of the functional plasticity of human Cytochromes P450. She joined Andrew Griffiths’ group in 2003, in the Laboratory of Molecular Biology (LMB) in the Medical Research Council in Cambridge (UK), to work on in vitro compartmentalization of biological and chemical reactions in emulsion droplets and participated to the development of High-throughput selection procedures of double-emulsions by FACS. In 2005, she integrated the Laboratory of Biological Chemistry directed by Pr. A. Griffiths in the ISIS institute (Univ. Strasbourg) as a permanent CNRS researcher to work on HTS procedures using droplet-based microfluidics. Since 2008, she develops droplet-based digital procedures for Cancer diagnosis. Recently, her team developed highly sensitive quantitative procedures for the detection of Cancer Biomarkers in droplets in a collaboration involving the clinical laboratory of Pr. P. Laurent-Puig (hEGP hospital, Univ. Paris-Descartes), the team of Dr J.-C. Baret (Max Plank Institute, Goettingen) and Raindance Technologies (US). She has recently created (January 2012) a new Group, named Translational Research And Microfluidics (TRAM), within a clinical oncology research unit in the university Paris-Descartes. Her research is dedicated to the clinical validation of the developed droplet-based procedures for the non-invasive detection of Cancer biomarkers, the highlighting of new Cancer Biomarkers and the development of original tools and procedures for their detection with applications in personalized medicine, cancer recurrence detection and cancer diagnostics.

 

PROFESSIONAL EXPERIENCES

Current Research position

CNRS Research Director (DR2) since 2015

Group Leader of the Team “Translational Research And Microfluidics” (http://recherche.parisdescartes.fr/UMRS775/Translational-Research-and-Microfluidics.) Paris-Descartes University, Paris – UMRS-1147 (ex. 775, Personalized medicine, pharmacogenomics, therapeutic optimization)- Director: Prof. P. Laurent-Puig.

Head of a “Ligue contre le cancer” labelized team since 2015

Teacher and coordinator of biology courses and internships in the “Frontier of Life, FDV” Bachelor, Paris Descartes University. http://www.licence.fdv-paris.org/en/licence-fdv/

Co-director of the eDIAG platform

2011-2015

CNRS researcher (CR1, permanent position) in the UMRS1147 (Pr P. Laurent-Puig), Paris Descartes University

Teacher and coordinator of biology courses and internships in the “Frontier of Life, FDV” Bachelor, Paris Descartes University. http://www.licence.fdv-paris.org/en/licence-fdv/

2006-2011

CNRS researcher (CR2 and then CR1) in the ISIS. UMR 7006, Strasbourg – Director: Prof. T. Ebbesen. Laboratory of Biological Chemistry (directed by Andrew Griffiths). 

Teaching in Master Degree in Université de Strasbourg for Toxicology and Molecular Biology (Micro-arrays and Biosensors).

2003-2006

Post-doctoral researcher. Team of Dr A. Griffiths. Medical Research Council, LMB, Cambridge, UK. "High throughput sorting of double-emulsions for directed evolution".

1999-2003

PhD in Molecular Toxicology. Supervision by Dr D. pompon. Fellow of french ministary of research, ligue contre le cancer and FRM.

Teaching as a « monitrice » in First year of medical studies and Bachelor. University Paris-Descartes (192 hours).

1997- 1998

Teaching for practical course in biochemistry (second year of medical studies), University Jules Verne (Amiens) and Teaching in a private institute to prepare first year students in medical and pharmaceutical studies (272 hours).

QUALIFICATIONS

2008

Habilitation to direct researches (HDR). "Protein directed evolution: from the creation of genetic diversity to the analysis within microreactors." University of Strasbourg.

2003

PhD of Molecular Toxicology. "Combinatorial approaches applied to the study of Cytochrome P450s functional diversity." University Paris Descartes (Paris V). French Research Ministary Fellowship and Teaching as a "Monitrice". Research performed in the Molecular Genetic Center (CNRS, Gif-sur-yvette). Supervisors: Dr Denis Pompon and Gilles Truan, UPR 2167.

1999

Master in Molecular Toxicology. University Paris Descartes. Major of promotion.

AWARDS, FELLOWSHIPS

2016-

Ligue contre le cancer labelized team (renewable for 4 years after evaluation)

2015

"Equipe à l’honneur" award ARC foundation (coord. P. Laurent-Puig, scientif. resp. V. Taly)

2012-2016

CNRS “Prime d’excellence scientifique” (PES).

2006

Long-term FEBS post-doctoral fellowship (declined for the CNRS permanent position).

2003-2006

Marie-Curie “Research Training Network” fellowship ENDIRPRO.

1998-2003

PhD Fellowships from the French Ministary of Research, Fondation pour la Recherche Medicale and Ligue contre le cancer

RESPONSABILITIES (selection)

Committees 

Nominated member of the scientific board of the Frontier of Life (FdV) doctoral School (ED474) since 2016

Nominated member of INSERM national scientific committee (CSS 6, “Technologies pour la santé”)  (2016-2021)

Permanent member of the Interdisciplinary panel fwo foundation (Belgium) (2015-2018)

Member of the steering committee of the micro and nanofluidic research network (GDR, CNRS) since 2015

Member of the scientific board of the UMRS1147 since 2011

Elected member of the scientific board of the Interdisciplinary Center for chemistry and biology/UFR biomédicale (2014-2016)

CNRS Observatory for Micro and Nano Technologies (OMNT, 2013-2014)

Conferences and workshops

Advisory panels: digital PCR & qPCR US 2016, Next-Generation Diagnostics Summit 2016 (US), Microfluidics Europe 2015, qPCR & digital PCR Europ 2014, Circulating Biomarkers US 2014.

MicroTas 2013 & 2014 Technical program committee

Member of International Scientific Committee (ISC) for NanoBio Surfaces and Interfaces in Healthcare and Science european workshop organization since 2016 (see http://corporateventuringnetwork.com/1401-2/)

Journals 

Analytical and Bioanalytical Chemistry, Biomicrofluidics, British Journal of cancer (BJC), Cancers, Cancer Research, Clinical Chemistry, Clinical Cancer Research, JoVe, Lab on a Chip, Microfluidics & Nanofluidics, Nature Protocols, Plos One, RSC advances, Scientific reports, etc.

Expertises for funding agencies 

French National Research Agency (ANR), European commission, Technology Foundation STW, Agence de la Biomedicine, Cancer Research UK, CNRS “mission pour l’interdisciplinarité” (Exomod), EADS foundation PhD price, etc.

Others 

Member of the « Female Mentoring program », University of Koln, Germany, 2014-2015

Member of the American association for cancer research (aacr) since 2011.

Member of the "research Group" GDR micro nano fluidics since 2011.

Member of the "research Group" GDR Protein Directed evolution. 2005-2011.

Member of the Innovating Teaching through Research Institute (IIFR, Center for Interdisciplinary Research, Paris).

EDITION ACTIVITIES

Microchips for diagnostics (2017) Co-edited book with Pr Stéphanie Descroix and Jean-Louis Viovy, Springer’s Methods in Molecular Biology (MiMB).

Invited editor for the special issue Digital PCR of Biomolecular Detection and Quantification journal (BDQ, Elsevier, 2016).

PUBLICATIONS (including book chapters, * corresponding authorship)

2017

Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics. S. Okumura, A. Baccouche, R. Sieskind, E. Henry, N. Aubert-Kato, N. Bredeche, J. F. Bartolo, V. Taly, Y. Rondelez, T. Fujii & A. J. Genot*. Nature Protocols (2017), 12(9):1912-1932.

Microfluidics as a strategic player to decipher single-cell omics? H. Lu, O. Caen, P. Nizard, V. Taly*. Trends in Biotechnology (2017), 35(8):713-727.

Functional Evidence for Derivation of Systemic Histiocytic Neoplasms from Hematopoietic Stem/Progenitor Cells. B. H Durham, D.Roos-Weil, C. Baillou, F. Cohen-Aubart, A. Yoshimi, M. Miyara, M. Papo, Z. Hélias-Rodzewicz, N. Terrones, N. Ozkaya, A. Dogan, R. Rampal, F. Urbain, L. Le Fèvre, E. L Diamond, C. Y Park, T. Papo, F. Charlotte, G. Gorochov, V. Taly, O. Bernard, Z. Amoura, O. Abdel-Wahab, F. M Lemoine, J. Haroche, and J-F. Emile*. Blood (2017), I130(2):176-180.

Early evaluation of circulating tumor DNA as marker of therapeutic efficacy in metastatic colorectal cancer patients (PLACOL study). F. Garlan, P. Laurent-Puig*, N. Siauve, A. Didelot, G. Perkins, H. Blons, J. Taieb, V. Taly* and A. Zaanan. Clinical Cancer Research (2017), In press. doi: 10.1158/1078-0432.CCR-16-3155.

High-throughput cell counting using droplet-based microfluidics. H. Lu, O. Caen, J. Vrignon, P. Nizard, J-C. Baret, V. Taly*. Scientific reports (2017), 7(1):1366.

Circulating cell-free BRAFV600E as a biomarker in children with Langerhans cell histiocytosis. Héritier S, Hélias-Rodzewicz Z, Lapillonne H, Terrones N, Garrigou S, Normand C, Barkaoui MA, Miron J, Plat G, Aladjidi N, Pagnier A, Deville A, Gillibert-Yvert M, Moshous D, Lefèvre-Utile A, Lutun A, Paillard C, Thomas C, Jeziorski E, Nizard P, Taly V, Emile JF, Donadieu J.* Br J Haematol. (2017), 178(3):457-467. doi: 10.1111/bjh.14695. 

Circulating tumor DNA Measurement by Picoliter droplet-based digital PCR and vemurafenib plasma concentrations in patients with advanced BRAF-mutated melanoma. Garlan F, Blanchet B, Kramkimel N, Puszkiel A, Golmard JL, Noe G, Dupin N, Laurent-Puig P, Vidal M, Taly V and Thomas-Schoemann A.* Targeted Oncology (2017), 12(3): 365-371.

A novel low cost method to prepare a cross-linked gelatin membrane for potential biological applications. Pitingolo, G.* and Taly, V. Lab on Chip, Chips and Tricks. (2017).

Droplet-Based Digital PCR: Application in Cancer Research. Perkins G, Lu H, Garlan F, Taly V.* Adv Clin Chem. (2017), 79:43-91.

Droplet-based Microfluidics digital PCR for the detection of KRAS mutations. Pekin D, Taly V.* Methods Mol Biol. (2017), 1547:143-164.

Multiplex detection of KRAS mutations using passive droplet fusion. Pekin D, Taly V.* Methods Mol Biol. (2017), 1547:133-142. 

Plasma Circulating Tumor DNA in Pancreatic Cancer Patients is a Prognostic Marker. Pietrasz D, Pécuchet N, Garlan F, Didelot A, Dubreuil O, Doat S, Imbert-Bismut F, Karoui M, Vaillant JC, Taly V, Laurent-Puig P, Bachet JB.* Clin Cancer Res. (2017), 23(1):116-123.

Edited Book:

Microchip Diagnostics. Methods in Molecular Biology. Methods and Protocols. Editors: Taly, Valérie, Viovy, Jean-Louis, Descroix, Stéphanie (Eds.)

Microchip for diagnostics

2016

Circulating tumor DNA to monitor first-line treatment in Non-Small Cell Lung Cancer patients, a prospective study using Base-Position Error Rate (BPER) analysis of Next-Generation Sequencing. N. Pécuchet, E. Zonta, A. Didelot, P. Combe, C. Thibault, L. Gibault, C. Luscan, Y. Rozenholc, V. Taly, P. Laurent-Puig, H. Blons*, E. Fabre. Plos Medecine (2016), 13(12):e1002199.

Editorial: Digital PCR, a technique for the future. Taly, V. and Huggett, J. Biomol Detect Quantif. (2016),10:1 

Germline and somatic mutations in MTOR gene in focal epilepsies with and without brain malformations. R. S. Møller*, S. Weckhuysen, M. Chipaux, E. Marsan, V. Taly, E. M. Bebin, S. Hiatt M, K. M. Bowling, D. Mei, V. Conti, P. de la Grange, S. Ferrand-Sorbets, G. Dorfmüller MD, V. Lambrecq, L. HG Larsen, E. Leguern, R. Guerrini, G. Rubboli, G.M. Cooper and S. Baulac. Neurology: Genetics (2016), 2:e118. 

Analysis of Base position Error rates analysis of next generation sequencing to detect circulating tumor DNA mutations. N. Pécuchet, Y. Rozenholc, E. Zonta, D. Pietraz, A. Didelot, J-B. Bachet, E. Fabre, V. Taly, H. Blons, P. Laurent-Puig*. Clinical Chemistry (2016), 62(11):1492-1503.

Multiplex detection of rare mutations by picoliter droplet based digital PCR : sensitivity and specificity considerations. E. Zonta, F. Garlan, N. Pecuchet, K. Perez-Toralla, C. Milbury, A. Didelot, O. Caen, H. Blons, E. Fabre, P. Laurent-Puig, V. Taly*. Plos One (2016), 11(7):e0159094.

BRAF mutation correlates with High-Risk Langerhans Cell Histiocytosis and increased resistance to first-line therapy. S. Héritier, J-F. Emile, M. Aziz-Barkaoui, C. Thomas, S. Fraitag, S. Boudjemaa, F. Renaud, A. Moreau, M. Peuchmaur, C. Chassagne-Clément, F. Dijoud, V. Rigau, D. Moshous, A. Lambilliotte, F. Mazingue, K. Kebaili, J. Miron, E. Jeziorski, G. Plat, N.  Aladjidi, A. Ferster, H. Pacquement, C. Galambrun, L. Brugières, G. Leverger, L. Mansuy, C. Paillard, A. Deville, C. Armari-Alla, A. Lutun, M. Gillibert-Yvert, J-L Stephan, F. Cohen-Aubart, J. Haroche, I. Pellier, F. Millot, B. Lescoeur, V. Gandemer, C. Bodemer, R. Lacave, Z. Hélias-Rodzewicz, V. Taly, F. Geissmann, and J. Donadieu*. Journal of Clinical Oncology, (2016), 34(25):3023-30.

High-resolution mapping of bifurcations in nonlinear DNA circuits. A.J. Genot, A. Baccouche, R. Sieskind, N. Aubert-Kato, N. Bredeche, J.F. Bartolo, V. Taly, T. Fujii, Y. Rondelez. Nature Chemistry (2016), 8(8):760-7.

A Study of Hypermethylated Circulating Tumor DNA as a Universal Colorectal Cancer Biomarker. S. Garrigou, G. Perkins, F. Garlan, C. Normand, A. Didelot, D. Le Corre, S. Peyvandi, C. Mulot, R. Niarra, P. Aucouturier, G. Chatellier, P. Nizard, K. Perez-Toralla, E. Zonta, C. Charpy, A. Pujals, C. Barau, O. Bouché, J.-F. Emile, D. Pezet, F. Bibeau, J. B. Hutchison, D. R. Link, A. Zaanan, P. Laurent-Puig, I. Sobhani and V. Taly*. Clinical Chemistry (2016), 62(8):1129-39.

2015

Quel avenir pour l’ADN tumoral circulant? Etat des lieux et perspectives dans les cancers colorectaux, pulmonaires non à petites cellules et pancréatiques. D. Pietrasz, N. Pécuchet, E. Fabre, H. Blons, V. Taly, P. Laurent-Puig, J-B Bachet*. Bulletin du cancer (2015), 103(1):55-65.

Why and how immunohistochemistry should now be used to screen for the BRAFV600E status in metastatic melanoma? The experience of a single institution (LCEP, Nice, France). Long E, Ilie M, Lassalle S, Butori C, Poissonnet G, Washetine K, Mouroux J, Lespinet V, Lacour JP, Taly V, Laurent-Puig P, Bahadoran P, Hofman V, Hofman P.* J Eur Acad Dermatol Venereol (2015) doi: 10.1111/jdv.13332. [Epub ahead of print]

Variations of BRAF mutant allele percentage in melanomas. Hélias-Rodzewicz Z, Funck-Brentano E, Baudoux L, Jung CK, Zimmermann U, Marin C, Clerici T, Le Gall C, Peschaud F, Taly V, Saiag P, Emile JF.* BMC Cancer (2015), 15:497.

Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Lim,  J., Caen, O., Vrignon, J., Konrad, M., Taly, V. and Baret, J-C.* Co-first name for Lim, J. and Caen, O. Biomicrofluidics (2015), 9(3):034101.

Assessment of DNA integrity, applications for cancer research. Zonta, E., Nizard, P. and Taly, V.* Advances in Clinical Chemistry. (2015), 70:197-246.

Digital PCR compartmentalization I. Single-molecule detection of rare mutations. Perez-Toralla, K., Pekin, D., Garlan, F., Bartolo, JF, Nizard, P., Laurent-Puig, P., Baret, JC, Taly, V.* Revue invitée (Français) pour le journal Medecine et Sciences (2015), 31(1):84-92.

Digital PCR compartmentalization II. Contribution for the quantitative detection of circulating tumor DNA. Caen, O., Nizard, P., Garrigou, S., Perez-Toralla, K. Zonta, E., Garlan, F., Laurent-Puig, P., Taly, V.* Revue invitée (Français) pour le journal Medecine et Sciences (2015), 31(2):180-6.

Microfluidics and Tumor DNA. Contribution of digital PCR (technical review in French, french title: Microfluidique et ADN tumoral. Apport de la PCR digitale). Nizard P, Krol A, Laurent-Puig P, Taly V.* Techniques de l’ingénieur (2015). In press.

Clinical relevance of KRAS-mutated sub-clones detected with picodroplet digital PCR in advanced colorectal cancer treated with anti-EGFR therapy. Laurent-Puig, P.*, Pekin, D., Normand, C., Kotsopoulos, SK, Nizard, P., Perez Toralla, K., Rowell, R., Olson, J., Srinivasan, P., Le Corre, D., Hor, T., El Harrak, Z., Li X., Link, D.R., Bouche, Emile, J-F., O., Landi, B., Boige, V., Hutchison, J.B. and Taly, V.* Clinical Cancer Research (2015), 21(5):1087-97.

2014

Recurrent RAS and PIK3CA mutations in Erdheim-Chester disease.  Emile, J-F., Diamond, E.L., Hélias-Rodzewicz, Z., Cohen-Aubart, F., Charlotte, F., Hyman, D.M., Kim, E., Rampal, R., Patel, M., Ganzel, C., Aumann, S., Faucher, G., Le Gall, C., Leroy, K., Colombat, M., Kahn, J-E., Trad, S., Nizard, P., Donadieu, J., Taly, V., Amoura, Z., Abdel-Wahab, O. and Haroch, J. Blood (2014), 124(19):3016-9.

Association of both Langerhans cell histiocytosis and Erdheim-Chester Disease linked to BRAFV600E mutation. A multicenter study of 23 cases. B Hervier, J Haroche, L Arnaud, F Charlotte, J Donadieu, A Néel, F, Lifermann, C Villabona, B Graffin, O Hermine, A Rigolet, C Roubille, E Hachulla,T Carmoi, M Bézier, V Meignin, M Conrad, L Marie, E Kostrzewa, JM Michot, S Barete, V. Taly, K Cury, JF Emile, Z Amoura. Blood (2014), 124 (7) : 1119-1126.

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics. Beneyton, T., Coldren, F., Baret, J-C., Griffiths, A.D. and Taly, V.* Analyst (2014), 139(13):3314-23. (Invited article for the themed issue on “Probe and Chip Approaches to Cell Analysis”).

The Microfluidic Puzzle: Chip-oriented Rapid Prototyping. Lim, J., Maes, F., Taly, V. and Baret, J-C. Lab on Chip (2014), 14 (10), 1669-1672.

2013

Circulating DNA, digital PCR and colorectal cancers. Taly, V and Laurent-Puig, P. Correspondances en Onco-theranostic (2013), 2(4), 184-189 (Invited review for the thematic issue : Cellules tumorales et ADN libre circulant). 

Multiplex picodroplet digital PCR to detect KRAS mutationsin circulating DNA from plasma of colorectal cancer patients. Taly, V.*, Pekin,D., Benhaim, L., Kotsopoulos, SK, Le Corre, D., Li X., Atochin, I., Link, D.R.,Griffiths, A.D., Pallier, K., Blons, H., Bouche, O., Landi, B., Hutchison, J.B.and Laurent-Puig, P. Clinical Chemistry (2013), 59(5):815-823.

High-throughput formation and control of monodisperse liquidcrystals droplets driven by an AC fields in a microfluidic device. Belloul, M.,Bartolo, J-F., Ziraoui, B., Coldren, F., Taly, V., El Abed, A.I.* AppliedPhysics Letters (2013), 103 (3), 033112. http://dx.doi.org/10.1063/1.4813880

Real-time detection of Whispering Gallery Mode resonance inhigh-throughput flowing monodisperse microdroplets. El Abed, A.I.* and Taly, V. Optical Materials (2013). 36(1): Pages 64–68.

Multiplex picoliter-droplet digital PCR for quantitativeassessment of DNA integrity in clinical samples. Didelot A, Kotsopoulos SK,Lupo A, Pekin D, Li X, Atochin I, Srinivasan P, Zhong Q, Olson J, Link DR,Laurent-Puig P, Blons H, Hutchison JB, Taly V. Clinical Chemistry (2013),59(5):815-823.

Membraneless Glucose/O2 microfluidic biofuel cells usingcovalently bound enzymes. Beneyton, T., Putu Mahendra Wijaya, I.  , C. Ben Salem, C., Griffiths, A.D. andTaly, V. Chemical communications (2013), 49(11): 1094-1096.

2012

Dynamics of molecular transport by surfactants in emulsions.Skhiri, Y., Gruner, P., Semin, B., Brosseau, Q., Pekin, D., Mazutis, L., Goust,V., Kleinschmidt, F., El Harrak, A., Hutchison, J. B., Mayot, E., Bartolo,J-F., Griffiths, A.D. , Taly, V.* and Baret, J-C.*. Soft Matter (2012), 8:10618-10627. Journal Cover. 

Detecting Biomarkers with microdroplet technology. Taly,V.*, Pekin, D, El Abed A. and Laurent-Puig, P. Invited review. Trends inMolecular Medicine. 2012 Jul;18(7):405-16. Journal cover.

2011 

Sensitive detection of rare mutations using droplet-basedmicrofluidics. Baret J.-C. and Taly V. G.I.T. Journal Laboratory Europe (2011),15: 12-13. 

Quantitative and sensitive detection of rare mutations usingdroplet-based microfluidics. Pekin, D., Skhiri, Y., Baret, J.-C., Le Corre, D.,Mazutis, L., Ben Salem, C., Millot, F., El Harrak, A., Hutchison, J.B., Larson,J.W., Link, D.R., Laurent-Puig, P., Griffiths, A.D., and Taly, V.* Lab on achip (2011), 11(13): 2156-66. Journal inside cover Picture. 

Highlights in: www.genomeweb.com

Press Release by CNRS, Université de Strasbourg, UniversitéParis-Descartes and Max Plank Institute (seehttp://www2.cnrs.fr/presse/communique/2187.htm and followed by Articles inFrench Press) as well as in the CNRS international Journal. Television (5) andradio (2) interviews.

Multiplex Digital PCR: Breaking the One Target Per Color Barrier of Quantitative PCR. Zhong,Q., Bhattacharya, S., Kotsopoulos, S., Olson, J., Taly, V., Griffiths, A.D.,Link, D.R., and Larson. J.W., Lab on a chip (2011), 11(13):2167-74.

Immobilisation of CotA, an extremophilic laccase fromBacillus subtilis, on glassy carbon electrodes for biofuel cell applications.Beneyton, T., El Harrak, A., Griffiths, A.D. and Taly, V.* ElectrochemistryCommunications (2011), 13(1): 24-27.

KRAS mutation detection trap. Benhaim, L., Maley, K., LeCorre, D., Blons, H., Taly, V., Bibeau, F., Emile, J-F. and Laurent-Puig, P.Journal of Clinical Oncology (2011), Letter, 29(8): e208-e209.

From Toxins Targeting Ligand Gated Ion Channels toTherapeutic Molecules. Nasiripourdori, A., Taly, V., Grutter, T. and Taly, A.Toxins (2011), 3(3): 260-293.

The thermophilic CotA laccase from Bacillus subtilis:Bioelectrocatalytic evaluation of O2 reduction in the direct and mediatedelectron transfer regime. Beneyton, T., Beyl, Y., Guschin, D., Griffiths, A.D.,Taly, V. and Schuhmann, W. Electroanalysis (2011), 23 (8): 1781-9.

2010 

Tiny droplets for high-throughput cell-based assays. Baret,J.-C. and Taly, V. In Unravelling more of cell genomics : let’s use the microand nanotools !, RCS publication edited by M.-C. Potier and N. Bontoux. (2010)261-84.

2009

Microfluidic fluorescent activated droplet sorting (mFADS)for biological applications. Baret, J.-C., Miller, O.J., Taly, V., El Harrak,A., Ryckelynk, M., Agresti, J., Weitz, D. and Griffiths A.D. Co-first name forBaret JC and Miller OJ. Lab on a Chip (2009), 9(13): 1850-8. Journal coverPicture.

Highlights in: "Highlights in Chemical Biology", rsc publishing. Cell sortingsorted (2009)

Droplets and Emulsions: very high-throughput in biology.Baret, J.-C., Taly, V., Ryckelynk, M. and Griffiths A.D. Medecine et sciences(2009), 25(6-7): 6. 

Droplet-based microfluidic systems for high throughputsingle DNA molecule amplification and analysis. Mazutis L., Fallah-Araghi, A.,Miller, O. J., Baret, J. C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J.,Hutchison, B. J., Link, D., Griffiths, A.D. and Ryckelynck, M. AnalyticalChemistry (2009), 81(12): 4813-21. 

Multi-step microfluidic droplet processing: kinetic analysisof an in vitro translated enzyme. Mazutis L., Baret, J. C., Treacy, P., Skhiri,Y., Fallah-Araghi, A., Ryckelynck, M., Taly, V.* and Griffiths, A.D.*  Lab on a Chip (2009), 9: 2902-8.

2007

Miniaturizing chemistry and biology in microdroplets. Kelly,B.T., Baret, J.C., Taly, V. and Griffiths, A.D. Chemical Communications(Cambridge, UK) (2007), 18: 1773-88.

A combinatorial approach to substrate discrimination in theP450 CYP1A subfamily. Taly, V., Urban, P., Truan, G. and Pompon, D. Biochim.Biophys. Acta. (2007), 1770(3): 446-57.

Droplets as microreactors for High-Throughput Biology. Taly,V., Kelly, B.T. and Griffiths, A.D. ChemBioChem. (2007), 8(3):263-72.

2006

Directed evolution by in vitro compartmentalization. Miller,O.J., Bernath, K., Agresti, J.J., Amitai, G., Kelly, B.T., Mastrobattista, E.,Taly, V., Magdassi, S., Tawfik, D.S. and Griffiths, A.D. Nature Methods (2006),3(7): 561.

2005

High-throughput screening of enzyme libraries: in vitroevolution of a beta-galactosidase by fluorescence-activated sorting of doubleemulsions. Taly, V., Mastrobattista, E., Chanudet, E., Treacy, P., Kelly, B.and Griffiths, A. Co-first name. Chemistry and Biology (2005), 12(12):1291-300.

Highlights in:

- Nature. Vol. 440. 2006. Cornish V.W. New and views.Catalytic competition for cells. pp.156-157.

- Nature Methods. Vol. 3(2). 2006. Tiny droplets make a bigsplash. Eisenstein M. pp.71.

- Chemistry and Biology. Vol. 12, 1255-1258, 2005. Previews.O'Hare H.M. and Jonhsson K. The laboratory in a Droplet.

2003

Microarray based method for combinatorial library sequencemapping and characterization. Abecassis V., Jaffrelo L., Rickman D., AggerbeckL., Herbert C., Truan G. and Pompon D. Biotechniques (2003), 34: 1272-1279. 

Sequence mapping of combinatorial libraries on macro- andmicro-arrays: experimental design of DNA arrays. Abecassis, V., Truan, G.,Jaffrelo, L., and Pompon, D. In Methods in Molecular Biology (2003), 231:189-198. Directed Evolution Library Creation: Methods and Protocols. HumanaPress edited by F. H. Arnold.

Producing chimeric genes with the CLERY method: use of invitro and in vivo recombination techniques. Abecassis, V., Pompon, D., andTruan, G. In Methods in Molecular Biology (2003), 231: 165-173. DirectedEvolution Library Creation: Methods and Protocols. Humana Press edited by F. H.Arnold.  

Sequence mapping of combinatorial libraries on macro- andmicroarrays: bioinformatic treatement of data. Pompon, D., Truan, G. andAbecassis, V. In Methods in Molecular Biology (2003), 231: 199-211. DirectedEvolution Library Creation: Methods and Protocols. Humana Press edited by F. H.Arnold. 

Computational methods for sequence mapping of largecombinatorial libraries and deduced sequence signatures. Abecassis V.,Aggerbeck L., Truan G. and Pompon D. Biotechniques (2003), 34: 1280-1286.

Exploration of natural and artificial sequence spaces:towards a functionnal remodelling of membrane-bound cytochrome P450s. AbecassisV., Urban P., Truan G. and Pompon D. Biocatalysis and Biotransformation (2003),21 (2): 55-66.

2001

Design and characterization of a novel “family-shuffling”technology adapted to membrane enzyme: application to P450s involved inxenobiotic metabolism. Abecassis, V., Pompon, D. and Truan, G. Advance inExperimental Medicine and Biology (2001), 500: 319-322.

2000

High efficiency family shuffling based on multi-step PCR andin vivo DNA recombination in yeast: statistical and functional analysis of acombinatorial library between human cytochrome P450 1A1 and 1A2. Abecassis V.,Pompon D. and Truan G. Nucleic Acid Research (2000), 28: e88

PATENTS

Fluorocarbon emulsion stabilizing surfactants. Holtze, C.,Guerra, R.E., Agresti, J., Weitz, D., El Harrak, A., Miller, O., Baret, J.C.,Taly, V., Merten, C., Ryckelynck, M., Griffiths, A.D. WO/2008/021123.

Compositions and methods for in vitro sorting of molecularand cellular libraries. Tawfik, D., Bernath, K. , Aharoni, A., Peisajovich, S.,Griffiths, A.D., Mastrobattista, E., Magdassis, S., Taly, V., Chanudet, E.WO/2005/049787.

Improved combinatorial libraries by recombination in yeastand analysis method. Abecassis, V., Pompon, D. and Truan, G. CNRS/AventisPharma. WO/2001/096555

PROCEEDING ARTICLES (peer-reviewed)

Prognostic value of circulating tumor DNA in advanced colorectal cancer patients: quantification of hypermethylated or mutant sequences using picoliter-droplet digital PCR. F. Garlan, P. Laurent-Puig, N. Siauve, A. Didelot, G. Perkins, H. Blons, J. Taieb, V. Taly, A. Zaanan. Proceedings: AACR 107th Annual Meeting 2015, Avril 16-20, 2016; New Orleans, US.

Recurent RAS and PIK3CA mutations in Erdheim-Chester disease and histiocytic sarcoma. J. Haroche, E.L. Diamond, J-F Emile, Z. Helias-Rodzewicz, F. Cohen-Aubart, R. Charlotte, D.M. Hyman, R. Rampal, M. Patel, C. Ganzel, S. Aumann, G. Faucher, C. Le Gall, K. Leroy, M. Colombat, J.E. Kahn, S. Trad, P. Nizard, P J. Donadieu, V. Taly, Z. Amoura, O. Abdel-Wahab. Pediatric Blood & cancer, 62(1), p. S9-S10

Circulating tumor DNA as a prognostic marker in colorectal cancer: Preliminary results of a prospective trial. Pierre Laurent-Puig, Olivier Bouché, Ralph Niarra, Pascaline Aucouturier, Leonor Benhaim, Bruno Landi, Anne Berger, Thierry Lecomte, Corinne Normand, Delphine Le Corre, Audrey Didelot, Karine Mallet, Karla Perez Toralla, Thevy Hor, Zakaria El Harrak, Gilles Chatellier, Brian Hutchison, Darren Link, Valerie Taly. Proceedings: AACR 106th Annual Meeting 2015, Avril 18-22, 2015; Philadelphia, US.

Assessing cancer drug resistance at the single-cell level. O. Caen, E. Zonta, H. Lu, J. Vrignon , P. Nizard, J-C. Baret and V. Taly The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. Novembre 2014.

High-resolution landscape of a molecular program. A.J. Genot, A. Baccouche, R. Sieskind, N. Aubert, N. Bredeche, J.F. Bartolo, V. Taly, T. Fujii, Y. Rondelez. Extended proceedings of DNA20 conference (2014) Kyoto, Japan.

Picoliter droplet-based digital PCR to quantify circulating DNA in plasma of metastatic colorectal cancer patients. Garlan F., Didelot A., Perkins G., Rice N., Zaanan A., Laurent-Puig P. and Taly V. The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. November 2014.  

Pipette-and-play: parallelized ultra-high throughput microfluidic emulsifier for quantitative biochemical assays. J. Lim, O. Caen, J. Vrignon, M. Konrad, V. Taly and J.C. Baret. In: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), p. 258-260. ISBN: 978-0-9798064-7-6.

Clinical significance of low frequency KRAS and BRAF subclones for advanced colon cancer management. Valerie Taly, Pierre Laurent-Puig, Deniz Pekin, Corinne Normand, Steve K. Kotsopoulos, Philippe Nizard, Karla Perez-Toralla, Rachel Rowell, Jeff Olson, Preethi Srinivasan, Delphine Le Corre, Thevy Hor, Zakaria El Harrak, Xinyu Li, Qun Zhong, Darren R. Link, Olivier Bouché, Jean-François Emile, Bruno Landi, Valérie Boige, J. Brian Hutchison. Proceedings: AACR 105th Annual Meeting 2014; Apr 5-9, 2014; San Diego. Published in Cancer Research October 1, 2014 ; 74:2820; DOI:10.1158/1538-7445.AM2014-2820. 

Quantitative cancer analysis using digital PCR: Absolute counting of DNA (solid tumors and liquid biopsies in glioma, breast, and colon cancer) and RNA (mRNA and miRNA using One-Step RT-dPCR). Michael L. Samuels, Valerie Taly, Leonora Balaj, Saumya Das, and Ben H. Park. Proceedings: AACR 105th Annual Meeting 2014, Avril 5-9, 2014; San Diego, CA. Published in Cancer Research, October 1, 2014 74:5316; DOI: 10.1158/1538-7445.AM2014-5316.

Peptide Nucleic Acid Fluorescent Probes For Detection Of Specific Biomarkers for digital microfluidics systems. Jean-Francois Bartolo, Sylain Ladame and Valerie Taly. The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. Novembre 2013.  

Detection and quantification of minoritary subclones of KRAS in metastatic colorectal cancers by digital microfluidics: therapeutic implications. D. Pekin, C. Normand, S. Kotsopoulos, X. Li, L. Benhaim, O. Bouché, T. Lecompte, D. Le Corre, T. Hor, Z. El Harrak, P. Nizard, D. Link, J.B. Hutschinson, P. Laurent-Puig and V. Taly. Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. Published in Cancer Research Avril 15, 2013; 73(8 Supplement): 4211. DOI:10.1158/1538-7445.AM2013-4211

Microfluidic Approaches for the Study of Emulsions: Transport of Solutes. Philipp Gruner, Yousr Skhiri, Benoit Semin, Quentin Brosseau, Andrew D Griffiths, Valérie Taly, Jean-Christophe Baret. MRS Proceedings / Volume 1530 / 2013, DOI: http://dx.doi.org/10.1557/opl.2013.80.

Picoliter droplet-based digital PCR for the quantitative andsensitive detection of rare mutations and the assessment of DNA integrity. Deniz Pekin, Audrey Didelot, Steve K. Kotsopoulos, Jean-Christophe Baret,Delphine Le Corre, Brian Hutchison, Darren R. Link, Pierre Laurent-Puig, AndrewD. Griffiths, and Valérie Taly. Proceedings of the 3rd European Conference onMicrofluidics - Microfluidics 2012 - Heidelberg, December 3-5, 2012

Quantitative detection of circulating tumor DNA bydroplet-based digital PCR. Valerie Taly, Deniz Pekin, Steve Kotsopoulos, XinyuLi, Ivan Atochin, Hu Gang, Delphine Le Corre, Leonor Benhaim, J. BrianHutchison, Darren R. Link, Hélène Blons, Pierre Laurent-Puig. AmericanAssociation for Cancer annual meeting (AACR 2012). March 31-April  4th, Chicago, USA.

Quantitative detection of circulating tumor DNA in plasmasamples by droplet digital PCR. Pekin D., Kotsopoulos S., Xinyu L.,Atochin I., Gang H., Le Corre D., Benhaim L., Hutchison J. B., Link D.R., Blons H., Laurent-Puig P. and Taly V.,* The 16th Internationalconference on miniaturized systems for Chemistry and Life Science (MicroTas2012). Oct. 28- Nov. 1, 2012. Okinawa. Japan.

Multiplex highly sensitive detection of cancer biomarkers inbiological samples. D. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis,L., Ben Salem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. In: Proceedings of the 15th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2011, p. 665-667. ISBN: 978-0-9798064-4-5.

Integrated microfluidic platform for directed evolution oflaccase for Biofuel cell applications. Skhiri, Y., Beneyton, T., El Harrak, A.,Griffiths, A.D. and Taly, V. In: Proceedings of the 14th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2010, p. 980-982. ISBN:978-0-9798064-3-8.

Droplet-based microfluidics for the quantitative detectionof rare mutations. D. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis,L., Ben Salem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. In: Proceedings of the 14th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2010, p. 58-60. ISBN:978-0-9798064-3-8.

Mining the functional sequence space of shuffled P450s.Urban, P., Truan, G., Abecassis, V., and Pompon, D. 7th European ISSX Meeting,2004, Published in Drug Metabolism Reviews, 36 (1) : 253.

Yeast as a versatile tool for functional expression,characterization and engineering of P450s and associated redox enzymes. Pompon,D., Abecassis, V., Truan, G., Urban, P. May 21.–26th 2002. FEBS course:“Cytochrome P450 systems: from structure to application”. Kranjska gora,Slovenija 

INVITED CONFERENCES AND SEMINARS

Droplet-based microfluidics for single cell and single molecule analysis. Statistical Challenges in Single-Cell Biology workshop, April 30 to May 5, 2017, Ascona, Switzerland

Droplet based digital PCR for cancer patient follow-up. qPCR dPCR & NGS 2017 event, 3 - 7 April 2017, Freising, Germany

Droplet-based microfluidics: towards a new tool for cancer research. ELRIG’s Research and Innovation (R&I) Conference, March 29-30 2017,Cambridge, UK

Digital PCR and circulating plasmatic DNA: a multi-cancers analysis. Annual meeting for Grant awardees of Merieux Institute. Les Pensières conference center, October 2016, Annecy, FR.

Digital PCR for cancer patient follow up. Diagnostics Summit 2016 - Circulating Biomarkers for Liquid Biopsy Development and Companion Diagnostics, 13th-14th October 2016, Singapore. 

Droplet based microfluidics for single cell analysis. 4th Annual Single Cell Analysis Congress 2016, 10 -11 November 2016, London, UK.

From droplet-based microfluidics to digital PCR : The development of a new tool for cancer patient follow-up. NanoBio Surfaces and Interfaces in Healthcare and Science Event, 27-28 September 2016, Twente, NL.

Droplet based digital PCR for cancer patient follow up. Precision medicine based on liquid biopsies "from detection to dissection" symposium, 15 -16 September 2016, Granada, Spain.

Droplet digital PCR, liquid biopsies and patient follow-up. qPCR and Digital PCR Congress USA, Philadelphia, USA, 11-12 July 2016.

Microfluidics for cancer research. IC-ANMBES 2016, Brasov, Romania, June 2016.

Circulating tumor DNA as a new tool to follow cancer patients. Ecole thématique Hétérogénéité des tumeurs, Provins, France, May 2016.

Microfluidics for cancer research: a new tool for patients’ follow up. Journées de la federation CICB, Université Paris Descartes, France, May 2016.

Using Droplets to Highlight and Follow Cancer Genetic Markers. Molecular Dx Europe meeting, Lisbon, April 2016.

Microfluidics for cancer research: a new tool for patients’ follow up. Journée commune UFR Maths informatique et UFR biomédicale, Univ. Paris Descartes, January 2016.

Détecter et quantifier l’ADN tumoral circulant pour le suivi des patients atteints de cancers. Journées Onco-pharmacologie, Institut Cochin, Paris, France, 4th December 2015.

Tracking circulating tumor DNA using Picoliter droplet-based digital PCR. dPCR conference, San Diego, US, 2-3 November 2015.

Picoliter droplet-based microfluidics for cancer research: from the detection of low frequency KRAS subclones within tumors to the analysis of liquid biopsies. Microfluidics, London, UK, 20-21 October 2015.

Picoliter droplet-based digital PCR for cancer patient follow-up. Biotechnica 2015, Personalized medicine and its impact in the Clinic, Hanover, Germany, 7-8 Octobrer 2015.

Droplet based microfluidics to follow cancer genetic marker in the blood. NanoBlood conference, Paris, France, 6 July 2015

Droplet based microfluidics for cancer research : from the detection of low frequency KRAS subclones within tumors to the analysis of liquid biopsies. Invited conference, Institut du cerveau et de la moelle épinière (ICM), Paris, France, 3 July 2015

Monitoring circulating tumor DNA using picoliter droplet based digital PCR. qPCR and digital PCR congress USA, San Diego, 25-26 June 2015

Picoliter Droplet-Based Digital PCR for the Quantitative Analysis of Circulating Tumor DNA for Cancer Patient Follow Up. Festival of genomics, Raindance Technologies Workshop, Boston, US, 22 June 2015

PCR en microcompartiments : intérêt pour la recherche clinique. Colloque DIM analytics " La miniaturisation des mesures : quels espoirs, quels challenges ?", Paris, France, 17 April 2015

Picoliter Droplet-Based Digital PCR for the Quantitative Analysis of Circulating Tumor DNA for Cancer Patient Follow Up. Molecular Diagnostics Europ conference, Lisbon, Portugal, 15-16 April 2015

Picoliter Droplet-Based Digital PCR and microRNA Quantification. Extracellular Markers summit, Cambridge, US, 19-20 March 2015

Picoliter droplet digital PCR for cancer patient follow-up : application to colorectal cancer. 2nd qPCR & Digital PCR Congress, London, UK, 20-21 October 2014

Picoliter droplet digital PCR for cancer research. Taly V. Invited conference. Biopathology symposium. Paris, France, 19-20 June 2014.

Tiny droplets to detect cancer biomarkers. Taly V. Invited conference. 5th International Conference on Biomarkers and Clinical Research.  University of Oxford, St Hilda's College - UK, April 15-17, 2014.

Droplet digital PCR as a new tool for cancer research. Taly V. Invited conference. Advances in qPCR & dPCR conference .  Barcelona, Spain. 14-15 May 2014. 

Picoliter Droplet-Based Digital PCR for Molecular Diagnostics. Taly V. Invited conference. Molecular Medicine Tri conference 2014. San francisco, USA. 9-14 February 2014.

On the Journey to Personalised Follow ups: Using Circulating Tumour DNA in Colorectal Cancer Patients. Taly V. Invited conference. Circulate Europe - Berlin, Germany. 18-20 february 2014. Additional authors: Aziz Zaanan, Hélène Blons, Audrey Didelot, Géraldine Perkins, Nadejda Rice, Fanny Garlan, Philippe Nizard, Julien TAIEB and P. Laurent-Puig.

L’apport de la microfluidique à la PCR numérique dans la détection des biomarqueurs. Taly V. Invited conference. Journées Internationales de Biologie-  Paris, France. 13-15 November 2013.

How Digital PCR Simplifies Screening for Multiple Cancer Biomarkers. Taly, V. Invited Raindance Digital PCR webinars, 16th October 2013.

Droplet-based microfluidics as a tool for personalizedmedicine. Taly V. Invited conference. Digital PCR conference. San Diego, US, October 6-9 2013.

Detection of low frequency alleles in patient samples bydigital PCR. Taly V. Invited conference. qPCR and dPCR congress. Lyon, France,September 2013.

Digital PCR strategies in the development and analysis ofmolecular biomarkers for personalized medicine. Taly V. Invited conference (onbehalf of Prof. P. Laurent-Puig). qPCR and dPCR congress. Lyon, France,September 2013.

Droplet-based microfluidics for cancer biomarker screening.Taly, V. Invited Conference. Societé de pharmaco-toxicologie cellulaire (SPTC)annual conference. Chatenay-Malabry. France. 6th June 2013.

Droplet-based microfluidic for cancer research: Applications for personalized medicine. Taly, V. Invited Seminar. Microfluidique, MEMS,Nanostructures (MMN group), Gulliver Lab, ESPCI. Paris. France. May 21 2013.

Droplet-based microfluidic for cancer research: Applicationsfor personalized medicine. Invited Seminar. Microfabrication and cell biologyclub. May 3rd 2013.

Microdroplets for High-throughput screening of biologicaland chemical reactions : towards the development of new procedures for cancerresearch. IMTCE Seminar. Paris. January 2013.

Detection of cancer biomarkers in microdroplets. Taly, V.Invited Speaker. The International Conference for Micro& Nanotechnologiesfor the Biosciences, NanoBioTech-Montreux, Switzerland. November 12-142012. 

Microfluidics, droplets and Cancer research. Taly, V.Invited Speaker. Kagawa University Mini-conference on Novel devices applicableto bio/clinical/medical fields, Takamatsu, Japan. November 2, 2012.

Droplet-based Microfluidics for the Highly SensitiveDetection of Cancer Biomarkers in Biological Samples. Taly, V. Invited Speaker.Euromediag International Convention, 5th July 2012. Montpellier. France.

Tiny droplets to fight cancer. Taly, V. Invited Seminar.Laboratory for Photonics and Nanostructures (LPN), 5th June 2012. Paris.France.

Droplet-based Microfluidics for the High SensitivityDetection of Cancer Biomarkers in Biological Samples. Taly, V. Invited Speaker.Nano and Microfluidics conference. 30-31 May 2012. Hamburg. Germany.

Tiny droplets to fight cancer. Taly, V. Invited Seminar.Institut Jacques Monod, Université Paris 7. 20th March 2012. Paris. France.

Droplet-based microfluidics for biological applications:From enzymatic screening to biomarker analysis. Taly, V. Invited Seminar.Twente University. 6 February 2012. Enschede. Netherland.

Multiplex highly sensitive detection of Cancer Biomarkers inbiological samples. D. Pekin, S. Kotsopoulos, H. Gang, Q. Zhong, J-C. Baret, D.Le Corre, A. El Abed, J. B. Hutchison, D. Link, J. Larson, A. Griffiths, P.Laurent-Puig and V. Taly. Invited by Raindance Technologies. Association ofMolecular Pathology (AMP) 2011 Annual meeting workshop. 19 November 2011,Dallas, US.

How microdroplets could help fighting Cancer. Invited Speaker.Scientific days of the Ecole Doctorale Biologie-Santé Nantes (France). 8November 2011. Sable d’Olonne. France.

Droplet-based microfluidic for the quantitative detection ofrare mutations. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis, L., BenSalem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. Invited Speaker. MicroTas Conference. 4-7 October2010. Groningen. Netherlands.

Microfluidics. Invited seminar, UMRS-775 (Paris Descartesuniversity). 14 June 2010. Etampes. France.

Tiny droplets for single-cell analysis: From enzymaticscreening to cell sorting. Lorentz workshop "micro- and nanofluidics forcell biology". Invited Speaker. 17-22 January 2010.

Minaturizing the Laboratory in Emulsion Droplets. Stepsevent 2009, Meeting for french, german and swiss scientists in life sciences(funding companies/researchers) organised by the Alsace BioValley cluster. 15thDecember 2009. Strasbourg. France. 

Tiny droplets for cell-based assays. 2nd German / French /European Meeting on Yeast and Filamentous Fungi. 28-30 May 2009. Strasbourg.France.

Microfluidic systems for High-throughput screening anddirected evolution. Conectus meetings between Alsacian Companies and ResearchLaboratories. 16th October 2008.

Miniaturizing laboratory in emulsion droplets: applicationto toxicology. Invited seminar in the laboratory of Pr. P. Laurent-Puig, INSERMUMR S775, University Paris Descartes, June 2008, Paris, France.

Miniaturizing the Laboratory in Emulsion Droplets. Taly V.and Griffiths A. Next generation Therapeutics. November 2006, Basel,Switzerland.

Directed evolution of enzymes using selection ofdouble-emulsions by FACS. Application to the design of biocatalysts for biofuelcell. Taly V. and Griffiths AD. Invited seminar in the laboratory of Pr. H.Goerish, November 2004, Technical University of Berlin, Germany.

OTHER ORAL COMMUNICATIONS (selection).

Droplet-based microfluidic for cancer research: Applicationsfor personalized medicine. Taly, V. Invited by Raindance Technologies. qPCR andNGS 2013 event. Freising, Germany. 18-22 march 2013.

Quantitative detection of circulating tumor DNA in plasmasamples by droplet digital PCR. Pekin D., Kotsopoulos S., Xinyu L., Atochin I.,Gang H., Le Corre D., Benhaim L., Hutchison J. B., Link D. R., Blons H.,Laurent-Puig P. and Taly V.* The 16th International conference on miniaturizedsystems for Chemistry and Life Science (MicroTas 2012). Oct. 28- Nov. 1, 2012.Okinawa. Japan.

Emulsion PCR in microfluidics for the quantitative detectionof rare mutations: Towards an application in cancer diagnostics. D. Pekin, Y.Skhiri, J-C. Baret, D. Le Corre, L. Mazutis, C. Ben Salem, A. El Abed, J. B.Hutchison, D. Link, J. Larson, A. Griffiths, P. Laurent-Puig and V. Taly. GDR2825- New Approaches in Protein Directed Evolution. October 11-12th 2011.Ax-les-thermes, France. 

Multiplex highly sensitive detection of Cancer Biomarkers inbiological Samples. D. Pekin, Y. Skhiri, J-C. Baret, D. Le Corre, L. Mazutis,C. Ben Salem, A. El Abed, J. B. Hutchison, D. Link, J. Larson, A. Griffiths, P.Laurent-Puig and V. Taly. MicroTas Conference. 2-6 October 2011. Seattle. USA.

Quantitative detection of rare mutations using droplet-basedmicrofluidics. Taly V. Interbio Scientific Workshop. Microfluidics, from singlemolecule to cell biology. June 29th 2011. Institut Européen de Chimie etBiologie. Bordeaux, France.

Emulsion PCR in microfluidics for the quantitative detectionof rare mutations: Towards an application in cancer diagnostics. D. Pekin, Y.Skhiri, J-C. Baret, D. Le Corre, L. Mazutis, C. Ben Salem, A. El Abed, J. B.Hutchison, D. Link, J. Larson, A. Griffiths, P. Laurent-Puig and V. Taly.Micro- and Nano-fluidic GDR. May 23-25th 2011. Lyon, France.

Communications within the CNRS "research Group" «Protein Directed evolution» (GDR EDP, 2825). 1-2 meetings per year since 2005(oral presentations).

Design and characterization of a novel “family-shuffling”technology adapted to membrane enzyme: application to P450s involved inxenobiotic metabolism. Abecassis V., Pompon D. and Truan G. July 2000. 6thInternational Symposium on Biological Reactive Intermediates. Paris, France.

New DNA-shuffling approaches adapted to membrane enzymes:Library analysis and mosaic structure characterization. Abecassis V., QuineyC., Pompon D. and Truan G. September 2001. International conference of P450. LaGrande-Motte, France.

Screening P450 library: Living cell, microsomal preparationor permeabilized cell? Abecassis V., Nahon P., Pompon D. and Truan G. Septembre2001. Workshop Cytochromes P450 in bioréactors. International conference ofP450. La Grande-Motte, France. 

Functional analysis of mosaic P450 generated byDNA-shuffling strategy. Abecassis V., Quiney C., Jaffrelo L., Simoes N., TruanG. and Pompon D. May 21. – 26th 2002. FEBS course: “Cytochrome P450 systems:from structure to application”. Kranjska gora, Slovenia. 

Directed evolution of enzymes using selection ofdouble-emulsions by FACS. Taly V., Mastrobattista E., Chanudet E., KhodaverdiV. and Griffiths A. October 2004. Meeting of the ENDIRPRO Research TrainingNetwork (5th Framework). Louvain-la-neuve, Belgium.