Valerie TALY

Valérie TALY (Abécassis)

valerie.taly<at>parisdescartes.fr

44 years old

Married, 2 Children

 

BIOGRAPHY

Dr Valerie Taly is a CNRS research director and group leader of the Translational Research And Microfluidics group within the MEPPOT team (Personalized medicine, pharmacogenomics and therapeutic optimization). She is deputy director of this team headed by Prof. P. Laurent-Puig in the Cordeliers Research Center (CRC). 

After graduation as a Molecular Toxicologist, Dr Valerie Taly (Abecassis) performed her PhD research at the Center of Molecular Genetics (CNRS, Gif-sur-Yvette, FR) on applications of combinatorial methods (generally used for proteins directed evolution) to the study of the functional plasticity of human Cytochromes P450. She joined Andrew Griffiths’ group in 2003, in the Laboratory of Molecular Biology (LMB) in the Medical Research Council in Cambridge (UK, dir. Pr. Greg Winter), to work on in vitro compartmentalization of biological and chemical reactions in emulsion droplets and participated to the development of High-throughput selection procedures of double-emulsions by FACS. In 2005, she integrated the Laboratory of Biological Chemistry directed by Pr. A. Griffiths in the ISIS institute (created by Pr JM Lehn and directed by Pr T. Ebbesen at that time, Univ. Strasbourg),  as a permanent CNRS researcher to work on HTS procedures using droplet-based microfluidics. Starting from 2008, she focussed on the development of droplet-based digital procedures for Cancer diagnosis. In particular, her team developed highly sensitive quantitative procedures for the detection of Cancer Biomarkers in droplets in a collaboration involving the clinical laboratory of Pr. P. Laurent-Puig (hEGP hospital, Univ. Paris-Descartes), the team of Dr J.-C. Baret (Max Plank Institute, Goettingen) and Raindance Technologies (US, now part of BIO-Rad). She has recently created (January 2012) a new Group, named Translational Research And Microfluidics (TRAM), within the clinical oncology research unit MEPPOT in the university Paris-Descartes. Her research is dedicated to the clinical validation of the developed droplet-based procedures for the non-invasive detection of Cancer biomarkers, the highlighting of new Cancer Biomarkers and the development of original tools and procedures for their detection with applications in personalized medicine, cancer recurrence detection and cancer diagnostics.

CURRICULUM VITAE (updated Oct. 2019)

SCIENTIFIC TRACK

93 scientific publications including 80 original articles (30 as corresponding author; > 3200 citations; h-index = 31 (Google scholar), average citation per item> 44, 4 journal covers, 4 of highly cited (Top 1%) papers), 7 other publications (in French or editorials), 6 book chapters, 1 book “Microchips for Diagnostics” (Springer) co-ed. Pr J-L. Viovy & S. Descroix, 1 editorial for Biomolecular Detection and Quantification, Elsevier (BDQ, invited editor)
4 patents awarded Europe & USA and 4 patent applications
> 93 Invited Talks including 73 in international Conference/Workshops
37 Master /Bachelor students advised, 8 PhD advised (2009-2016), 3 on-going PhDs (in 1st, 2nd and 3rd year PhD), 11 Post-doc. supervised (3 still in the TRAM team), 1 permanent research engineer (present in the lab since 2012), 6 technicians or engineers supervised since 2011 (1 currently in the team).
Co-founder emulseo (https://www.emulseo.com/) start-up company

RESEARCH ACADEMIC POSITIONS

2019-today  Deputy director MEPPOT team (Team 26, Cordeliers research center, UMRS1138)
2016- today  Head of labelized Ligue contre le Cancer (LNCC) team
2015- today  CNRS Research director (DR2)
2012- today  Group leader of the “Translational Research And Microfluidics”-Univ. Paris-Descartes - UMRS-1147- Director: Prof. P. Laurent-Puig (now UMRS1138)
2006-2011 CNRS permanent Researcher (CR1 2010-2015). ISIS. UMR 7006, Strasbourg – Dir.: Prof. T. Ebbesen. Laboratoire de biologie chimique (dir.: Pr A. Griffiths).
2003-2006  Post-doctoral researcher. Team of Dr A. Griffiths. Medical Research Council, LMB, Cambridge, UK. "High throughput sorting of double-emulsions for directed evolution".
1999-2003  PhD student in molecular toxicology. Supervised by Dr D. Pompon and G. Truan. Funding from French ministry of research (MNERT), FRM and Ligue nationale contre le cancer (4th year).

TEACHING, MENTORING AND TRAINING ACTIVITIES

2017-  EMBL workshops on liquid biopsies and digital PCR
2011-  Frontiers of Life Sciences (FDV) Bachelor/ Univ. Paris-Descartes. Internships (~70h/yr) & Biology course coordination. Microfluidics Practical courses, FDV selection committee
2014-15   Online course/scientific flyer “liquid biopsy” for MDs (Accès cibles, Boehringer Ingelheim)
2014-15    Female Mentoring program/ Univ. Cologne/ Germany
2006-11  Master Degree - Toxicology and Molecular Biology (8h/yr), Univ. de Strasbourg / FR
1999-02    Teaching in 1rst yr of medical studies and Bachelor (192h), Univ. Paris-Descartes
1997-98   Practical courses Med. studies, Univ. Amiens & Training 1rst yr medical Exam (272h).

QUALIFICATIONS

2008  Habilitation à diriger les recherches (HDR). "Protein directed evolution: from the creation of genetic diversity to the analysis within microreactors." University of Strasbourg.
2003  PhD in Molecular toxicology. "Combinatorial approaches applied to the study of Cytochrome P450s functional diversity." University Paris Descartes (Paris V).
1999  Master of molecular toxicology. University Paris Descartes.

PRIZES/AWARDS/ACADEMY MEMBERSHIPS (2003-now)

2016-2019   CNRS award for supervision & research (“Prime d’encadrement et de recherche”, PEDR ex. PES).
2016-            Labelization from “ligue nationale contre le cancer” (renewable 4 times after evaluation)
2016           Prize “Equipe à l’honneur”, “Association for cancer research” (10k€, with P. Laurent-Puig)
2015   Advanced Merieux Institute Grant (co-coord. with P. Laurent-Puig)
2012-2015   CNRS award for scientific excellence (“Prime d’excellence scientifique”, PES).
2006     Long-term FEBS post-doctoral fellowship (3 yrs, declined for the CNRS position).
2003-2006  Marie-Curie “Research Training Network” Fellowship ENDIRPRO.

INSTITUTIONAL RESPONSIBILITIES/ COMMISSIONS OF TRUST

2018- today    Member of the working group of the French society of clinical biology (SFBC) on ddPCR2018- today  Scientific committee of the CARPEM SIRIC network, responsible of the axe 2 “technological developments”
2017- today  Partner steering Committee member of the ELICIT (Empowering LIfe sCiences with Innovative Technologie) DIM (Major Interest Domains, Ile-de-France region)
2017- today    Expert for the High Council for Evaluation of Research and Higher Education (HCERES, laboratory/Institute evaluations)
2017- today    Member of the founding committee of the « INTERNATIONAL SOCIETY OF LIQUID BIOPSY (ISLB) » (Spain, Granada, initiative of the Genyo Center Merck/Univ. Granada)
2016- today    Nominated member of the INSERM commission CSS6 Panel (Nominated member, Technologies for Health and public health, selection new researchers/research director, evaluation of researchers and research units)
2016- today    Nominated member of the Frontiers of Life Sciences (FDV) doctoral school (ED474) steering committee
2016- today    FWO (Fonds Wetenschappelijk Onderzoek) Interdisciplinary Expert panel member
2015- today    Board of the GDR “Micro nanofluidics” (CNRS research network)
2014- today    UMR S1147 scientific committee/ Univ. Paris-Descartes
2014-16     Scientific Advisory Board, Interdisdisciplinary Center for Chemistry and Biology (FR 3567, 2013-2016)
2013-15    Expert member for the https://www.omnt.fr/en/ (OMNT, CNRS)

ORGANISATION AND ADVISORY PANELS OF SCIENTIFIC MEETINGS

Congress advisory panels: Panel Chair/organisation SLAS 2019 (Washington 2019), 9th Annual NGS UK Congress & 5th Annual Single Cell Analysis UK Congress (2017), 3rd Annual NGS & Single Cell Analysis USA Congress (2017), digital PCR & qPCR US 2016, Next-Generation Diagnostics Summit 2016 (US), Microfluidics Europe 2015 (London), qPCR and dPCR world congress 2015 (Philadelphia), Circulating Cancer Biomarkers US 2014 (Boston), qPCR and digital PCR congress 2014 (London), Circulating Biomarkers US 2014, MicroTas 2013-15 (Technical Prog. Committee, TPC) and Flow17 TPC

Workshop organizations: Single cell genomics workshop (ICM institute, Paris) (2017); International Scientific Committee (ISC) NanoBio Interfaces in Healthcare and Sciences (2015-now, 1 meeting/yr)

REVIEWING/EXPERTISES/EDITING ACTIVITIES

Journals: Analytical Bioanalytical Chemistry, Analytical Chemistry, British Journal of Cancer, Cancers, Cancer Research, Clinical Chemistry, Clinical Cancer Research, Gut, IUBMB life, JoVe, Lab on a Chip, Microfluid Nanofluidics, Molecular Applications of Medicine, Nature Protocols, Plos One, RSC advances, Scientific Report, etc.

Institutions/Grant/Prize: “Association for cancer research (ARC)” foundation, Biomedicine agency, Canceropole "Aquitaine", Canceropole “Ile-de-France”, Canceropole "Grand-est", Canceropole "Auvergne-Rhone-Alpes", Campus France, Cancer Research UK, CNRS “mission for interdisciplinarity”, Dutch Cancer Society (KWF), EADS foundation PhD award (2011), European commission (Research and Innovation action (Research and Innovation Actions-RIA, ICT-03-2016); Future Emerging Technology (FET, member of follow-up committee 2015/17, project Ribonets), Marie-Curie Fellowship, Prestige Marie-Curie Fellowship); French National Research Agency (ANR), French National Cancer Institute (INCa), Idex Paris-Saclay, Kom op tegen Kanker (stand up to cancer, the Flemish cancer society), Ligue Nationale contre le cancer (LNCC), Science & Technology Foundation (STW, Belgium), Rosetrees Trust (UK, Interdisciplinary Prize Award), etc.

Editing: Board Editor Scientific Report since 2018, Invited editor (with Dr D. Pekin) of Micromachine journal special issue "Microfluidics-based Liquid Biopsies" (publication targeted in Sept. 2019), Co-ed. Pr S Descroix/JL Viovy "Microchips for diagnostics" (2017), Springer, Methods in Molecular Biology (MiMB); Guest editor with Pr J Huggett, Biomol. Detect. Quantif. (BDQ, 2016), Elsevier.

Other committee/Jurys: Jury of selection of new students for the FdV Bachelor and doctoral school “Frontiers of innovation in research (ED474) (2017 & 2019), University Paris Descartes since 2012; Member of the evaluation committee “Physics for cancer” 2018 (Research projects in physics, mathematics or engineering sciences related to Cancer); Vice-chair of the committee of evaluation PCSI 2019 (“interdisciplinary approaches of oncogenic processes and therapeutic perspectives: benefit for oncology of physics, chemistry and ingeniering sciences”, INSERM Cancer plan)

Participation to jurys: University full professor position, University of Strasbourg (May 2019); University assistant professor position, ESBS engineer school, University of Strasbourg (April 2018); Junior Group Leader, University of Leuven (Belgium, December 2018),  19 PhD jury since 2013 (5 abroad) including 14 as reviewer or opponent and 2 as president of the jury.

Participation to evaluations of laboratories or institutes: Member of HCERES committees with on-site visits since 2016; Evaluation for Cancer Research UK (2018); Evaluation of INSERM research units since 2016

 

PUBLICATIONS (including book chapters, * corresponding authors)

2019

[Development of molecular analysis by digital PCR for clinical practice: positionning, current applications and perspectives.] J.A. Denis, A. Perrier, J. Nectoux, P-J. Lamy, A-S. Alary, N. Sarafan-Vasseur, D. Hennaff, B. Busser, R. Appay, P. Pedini, P. Romanet, V. Taly, F. Fina. For the working group of the french society of clinical biology “digital PCR”. Annales de biologie clinique (2018), Review in French, in press. IF: 0.5

HPV circulating tumoral DNA quantification by droplet-based digital PCR: a promising predictive and prognostic biomarker for HPV-associated oropharyngeal cancers. D. Veyer, M. Wack, M. Mandavit, S. Garrigou, S. Hans, P. Bonfils, L. Belec, S. Wang-Renault, P. Laurent-Puig, H. Mirghani, B. Rance, V. Taly, C. Badoual, H. Péré*. International Journal of Cancer (2019), in press. IF: 7.36

Molecular program for isothermal digital detection of microRNA. G. Gines*, R. Menezes*, N. Kaori, A-S. Kirstetter,  V. Taly* and Y. Rondelez*. bioRxiv preprint first posted online Jul. 14, 2019, doi: https://doi.org/10.1101/701276 - Science Advances (2019), in press. IF: 12.804

Plasma clearance of RAS mutation under therapeutic pressure is a rare event in metastatic colorectal cancer. E. Moati, H. Blons, V. Taly, F. Garlan, S. Wang-Renault, D. Pietrasz, A. Didelot, G. Sonia, A. Saint, S. Pernot, J. Taieb, P. Laurent-Puig, A. Zaanan*. International Journal of Cancer (2019), Doi: 10.1002/ijc.32657. [Epub ahead of print] IF: 7.36

Mechanical characterization of cells and microspheres sorted by acoustophoresis with in-line resistive pulse sensing. A Riaud*, ALP Thai, W Wang, V Taly. arXiv preprint posted online Jun 2019, arXiv:1906.11944

BRAF mutation analysis in circulating tumor DNA from patients with  metastatic colorectal cancer: complementary results from the AGEO RASANC  study. L. Mas, J-B. Bachet, V. Taly, O. Bouche, J.  Taieb, R. Cohen, A. Meurisse, C. Normand, J-M. Gornet,  P. Artru, S. Louafi, A. Thirot-bidault, I. Baumgaertner, R.  Coriat, D. Tougeron, T. Lecomte, F. Mary, T. Aparicio,  L. Marthey, H. Blons, D. Vernerey, P. Laurent-Puig*. Cancers (2019), 11(7). pii: E998. IF: 5.326

Digital PCR: principle and applications. Poulet G. and Taly V*. Onco-theranostic (2019), Invited review in French, In press.

Vemurafenib in refractory multisystem Langerhans cell histiocytosis (LCH) of children: results of an international observational study. J. Donadieu, I. A. Larabi, M. Tardieu, J. Visser, H. Caroline, E. Sieni, N. Kabbara, M. Barkaoui, P. Milne, J. Haroche, Z. Helias, M. Jehanne, A. Kolenova, A. Pagnier, N. Aladjidi, P. Schneider, G. Plat, A. Lutun, A. Heisig, A. Ferster, V.  Efremova, M. Ahlmann, L. Blanc, J. Nicholson, A. Lambilliote, H. Boudiaf, A. Lissat, K. Svojgr, F. Bernard, S. Elitzur, M. Golan, D. Evseev, M. Maschan, A. Idbaih, O. Slater, M. Minkow, V. Taly, M. Collin, J-C. Alvarez, J-F. Emile, S. Héritier*. Journal of Clinical Oncology (2019). In press. IF: 26.3

Tunable and reversible gelatin-based bonding for microfluidic cell culture. G. Pitingolo*, P. Nizard, A. Riaud and V. Taly*. Advanced Engineering Materials (2019). 1900145. IF: 2.360

Gelatin Coated Microfluidic Channels for 3D Microtissue Formation: on-Chip Production and Characterization. G. Pitingolo*, P. Nizard, A. Riaud, C.  Nastruzzi and V. Taly*. Micromachines (2019),10(4). IF: 1.8 

Coins in microfluidics: from mere scale objects to font of inspiration for microchannel circuits. G. Pitingolo*, V. Taly  and C. Nastruzzi. Biomicrofluidics (2019), 13(2):024106. IF: 2.57 

Liquid biopsy: general concepts. G. Poulet, J. Massias and V. Taly*. Invited review, Acta cytologica (2019), pp. 1-7. IF: 0.88 

HPV-circulating tumoural DNA by droplet-based digital polymerase chain reaction, a new molecular tool for early detection of HPV metastatic anal cancer? A case report. D. Veyer, J. Pavie, S. Pernot, M. Mandavit, S. Garrigou, M-L. Lucas, L. Gibault, V. Taly, L. Weiss, H. Péré*. European journal of cancer (2019), 112:34-37. IF: 5.4 

Microfluidic extraction of circulating tumor DNA for the sensitive detection and quantification of rare mutations. K. Perez-Toralla, I. Pereiro, J. Champ, F. Faqir, P. Nizard, L. Malaquin, J-L. Viovy, V. Taly* and S. Descroix*. Sensors and Actuators B: Chemical (2019), 286: 533-539. IF: 6.393

Highly sensitive methods are required to detect mutations in histiocytoses. S. Melloul, Z. Hélias-Rodzewicz, F. Cohen-Aubart, F. Charlotte, S. Fraitag, N. Terrones, Q. Riller, T. Chazal, S. Heritier, A. Moreau, M. Kambouchner, MC. Copin, J. Donadieu, V. Taly, Z. Amoura, J. Haroche, JF Emile*. Haematologica (2019), 104(3): e97-e99. IF: 9.09

2018

Beyond the on/off chip trade-off: A reversibly sealed microfluidic platform for 3D tumor microtissue analysis.  G. Pitingolo*, P. Nizard, A. Riaud and V. Taly*. Sensors and Actuators B: Chemical (2018), 274: 393-401. IF: 6.39

Incidence and risk factors for clinical neurodegenerative Langerhans cell histiocytosis: a longitudinal cohort study. S. Héritier, M-A. Barkaoui, J. Miron, C. Thomas, D. Moshous, A. Lambilliotte, F. Mazingue, K. Kebaili, E. Jeziorski, G. Plat, N. Aladjidi, H. Pacquement, C. Galambrun, L. Brugières, G. Leverger, L. Mansuy, C. Paillard, A. Deville, A.  Pagnier, A. Lutun, M. Gillibert-Yvert, J-L. Stephan, F. Cohen-Aubart, J. Haroche, I. Pellier, F. Millot, V. Gandemer, N. Martin-Duverneuil,V. Taly, Z. Hélias-Rodzewicz, J-F. Emile,  K. Hoang-Xuan, A.  Idbaih,  J. Donadieu*. The Brittish journal of haematology (2018), 183(4): 608-617. IF: 5.67

High throughput multiplexed fluorescence activated droplet sorting. Ouriel Caen, Simon Schuetz, Mani Jammalamadaka, Jeremy Vrignon, Philippe Nizard, Tobias Schneider, Jean-Christophe Baret* and Valérie Taly* Microsystems & Nanoengineering (Recent journal of Nature group) (2018), UNSP 33.  IF: 6

[Development of digital PCR molecular tests for clinical practice: principles, practical implementation and recommendations.] J.A. Denis, J. Nectoux, P-J. Lamy, C. Rouillac Le Sciellour, H. Guermouche, A-S. Alary, O. Kosmider, N. Sarafan-Vasseur, C. Jovelet, B. Busser, P. Nizard, V. Taly, F. Fina. For the working group of the french society of clinical biology “digital PCR”. Annales de biologie clinique (2018), Review in French, 76(5):505-523. IF: 0.5

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine. AS Whale, GM Jones, J. Pavšič, T. Dreo, N. Redshaw, S. Akyürek, M. Akgoz, C. Divieto, M.P. Sassi, H-J. He, K. D. Cole, Y-K. Bae, (…), L. Deprez, P. Corbisier, S. Garrigou, V. T., R. Larios, S. Cowen, D. M. O’Sullivan, C. A. Bushell, H. Goenaga-Infante, H. Parkes, C. A. Foy, A. J. Woolford, J. F. Huggett*, A. S. Devonshire*. Clinical Chemistry (2018), 64(9):1296-1307. IF: 8.08

Role of circulating tumor DNA in the management of patients with colorectal cancer. Moati, E., Taly, V., Didelot, A., Perkins, G., Blons, H., Taieb, J., Laurent-Puig, P., Zaanan, A*. (Review) Clinics and research in hepatology and gastroenterology. (2018), 42(5) : 396-402. IF: 2.6

BRAF V600E mutation detected in a case of Rosai-Dorfman disease. G. Fatobene, J. Haroche, Z. Hélias-Rodzwicz, F. Charlotte, V. Taly, A. Meneses Ferreira, A. Néder Ramires Abdo, V. Rocha, J-F. Emile*. Haematologica (2018), 103(8):e377-e379. IF: 9.09

*Mutation and methylation analysis of circulating tumor DNA can be used for the follow-up of metastatic colorectal cancer patients. N. Boeckx, K. Op de Beeck, M. Beyens, V. Deschoolmeester, C. Hermans, P. Declercq, K. Papadimitriou, P. Laurent-Puig, P. Pauwels, Guy Van Camp*, V. Taly*, M. Peeters*. Clinical Colorectal Cancer (2018), 7(2):e369-e379. IF: 2.8

BIABooster: Online DNA Concentration and Size Profiling with a Limit of Detection of 10 fg/μL and Application to High-Sensitivity Characterization of Circulating Cell-Free DNA. CL. Andriamanampisoa, A. Bancaud, A.  Boutonnet-Rodat, A. Didelot, J. Fabre, F. Fina, F. Garlan, S. Garrigou, C. Gaudy, F. Ginot*, D. Henaff, P. Laurent-Puig, A. Morin, V. Picot, L. Saias, V. Taly, P. Tomasini, A. Zaanan. Analytical Chemistry (2018), 90(6): 3766-3774. IF: 6.3

Chips and Tips: A second life for old electronic parts: a spin coater for microfluidic applications. Pitingolo, G., Taly, V. and Nastruzzi, C. Lab on Chip (2018)

RAS mutation analysis in circulating tumor DNA from patients with metastatic colorectal cancer: the AGEO RASANC prospective multicenter study. J. B. Bachet, O. Bouché, J. Taieb, O. Dubreuil, M. L. Garcia, A. Meurisse, C. Normand, J. M. Gornet, P. Artru, S. Louafi, F. Bonnetain, A. Thirot-Bidault, I. Baumgaertner,  R. Coriat, D. Tougeron,  T. Lecomte, F. Mary,  T. Aparicio,  L. Marthey, V. Taly, H. Blons,  D. Vernerey, P. Laurent-Puig.* Annals of oncology (2018), 29(5):1211-1219. IF: 13.9

Phenotypes and survival in Erdheim-Chester disease: Results from a 165-patient cohort. American Journal of Heamatology (2018), 93(5): E114-E117. IF: 5.3

2017

Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations. Pekin D, Taly V.*  Methods Mol Biol. (2017), 1547:143-164.

Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion. Pekin D, Taly V.* Methods Mol Biol. (2017), 1547: 133-142.

Droplet-based digital PCR and Next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective. M. Postel#, A.  Roosen #, P. Laurent-Puig, V. Taly* S. Wang-Renault*. Expert Review of Molecular Diagnostics (2017), 18(1):7-17. IF: 3.32

Functional Evidence for Derivation of Systemic Histiocytic Neoplasms from Hematopoietic Stem/Progenitor Cells. B. H Durham, D.Roos-Weil, C. Baillou, F. Cohen-Aubart, A. Yoshimi, M. Miyara, M. Papo, Z. Hélias-Rodzewicz, N. Terrones, N. Ozkaya, A. Dogan, R. Rampal, F. Urbain, L. Le Fèvre, E. L Diamond, C. Y Park, T. Papo, F. Charlotte, G. Gorochov, V. Taly, O. Bernard, Z. Amoura, O. Abdel-Wahab, F. M Lemoine, J. Haroche, and J-F. Emile. Blood (2017), 130(2):176-180. IF: 13.1

Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics. S. Okumura, A. Baccouche, R. Sieskind, E. Henry, N. Aubert-Kato, N. Bredeche, J. F. Bartolo, V. Taly, Y. Rondelez, T. Fujii & A. J. Genot*. Nature Protocols (2017), 12(9):1912-1932.

Microfluidics as a strategic player to decipher single-cell omics? H. Lu, O. Caen, P. Nizard, V. Taly*. Trends in Biotechnology (2017), 35(8):713-727.

Early evaluation of circulating tumor DNA as marker of therapeutic efficacy in metastatic colorectal cancer patients (PLACOL study). F. Garlan, P. Laurent-Puig*, N. Siauve, A. Didelot, G. Perkins, H. Blons, J. Taieb, V. Taly* and A. Zaanan. Clinical Cancer Research (2017), In press. doi: 10.1158/1078-0432.CCR-16-3155.

High-throughput cell counting using droplet-based microfluidics. H. Lu, O. Caen, J. Vrignon, P. Nizard, J-C. Baret, V. Taly*. Scientific reports (2017), 7(1):1366.

Circulating cell-free BRAFV600E as a biomarker in children with Langerhans cell histiocytosis. Héritier S, Hélias-Rodzewicz Z, Lapillonne H, Terrones N, Garrigou S, Normand C, Barkaoui MA, Miron J, Plat G, Aladjidi N, Pagnier A, Deville A, Gillibert-Yvert M, Moshous D, Lefèvre-Utile A, Lutun A, Paillard C, Thomas C, Jeziorski E, Nizard P, Taly V, Emile JF, Donadieu J.* Br J Haematol. (2017), 178(3):457-467. doi: 10.1111/bjh.14695. 

Circulating tumor DNA Measurement by Picoliter droplet-based digital PCR and vemurafenib plasma concentrations in patients with advanced BRAF-mutated melanoma. Garlan F, Blanchet B, Kramkimel N, Puszkiel A, Golmard JL, Noe G, Dupin N, Laurent-Puig P, Vidal M, Taly V and Thomas-Schoemann A.* Targeted Oncology (2017), 12(3): 365-371.

Chips and Tips: A novel low cost method to prepare a cross-linked gelatin membrane for potential biological applications. Pitingolo, G.* and Taly, V. Lab on Chip, Chips and Tricks. (2017).

Droplet-Based Digital PCR: Application in Cancer Research. Perkins G, Lu H, Garlan F, Taly V.* Adv Clin Chem. (2017), 79:43-91. 

Droplet-based Microfluidics digital PCR for the detection of KRAS mutations. Pekin D, Taly V.* Methods Mol Biol. (2017), 1547:143-164.

Multiplex detection of KRAS mutations using passive droplet fusion. Pekin D, Taly V.* Methods Mol Biol. (2017), 1547:133-142. 

Plasma Circulating Tumor DNA in Pancreatic Cancer Patients is a Prognostic Marker. Pietrasz D, Pécuchet N, Garlan F, Didelot A, Dubreuil O, Doat S, Imbert-Bismut F, Karoui M, Vaillant JC, Taly V, Laurent-Puig P, Bachet JB.* Clin Cancer Res. (2017), 23(1):116-123.

Edited Book:

Microchip Diagnostics. Methods in Molecular Biology. Methods and Protocols. Editors: Taly, Valérie, Viovy, Jean-Louis, Descroix, Stéphanie (Eds.)

Microchip for diagnostics

2016

Circulating tumor DNA to monitor first-line treatment in Non-Small Cell Lung Cancer patients, a prospective study using Base-Position Error Rate (BPER) analysis of Next-Generation Sequencing. N. Pécuchet, E. Zonta, A. Didelot, P. Combe, C. Thibault, L. Gibault, C. Luscan, Y. Rozenholc, V. Taly, P. Laurent-Puig, H. Blons*, E. Fabre. Plos Medecine (2016), 13(12):e1002199.

Editorial: Digital PCR, a technique for the future. Taly, V. and Huggett, J. Biomol Detect Quantif. (2016),10:1 

Germline and somatic mutations in MTOR gene in focal epilepsies with and without brain malformations. R. S. Møller*, S. Weckhuysen, M. Chipaux, E. Marsan, V. Taly, E. M. Bebin, S. Hiatt M, K. M. Bowling, D. Mei, V. Conti, P. de la Grange, S. Ferrand-Sorbets, G. Dorfmüller MD, V. Lambrecq, L. HG Larsen, E. Leguern, R. Guerrini, G. Rubboli, G.M. Cooper and S. Baulac. Neurology: Genetics (2016), 2:e118. 

Analysis of Base position Error rates analysis of next generation sequencing to detect circulating tumor DNA mutations. N. Pécuchet, Y. Rozenholc, E. Zonta, D. Pietraz, A. Didelot, J-B. Bachet, E. Fabre, V. Taly, H. Blons, P. Laurent-Puig*. Clinical Chemistry (2016), 62(11):1492-1503.

Multiplex detection of rare mutations by picoliter droplet based digital PCR : sensitivity and specificity considerations. E. Zonta, F. Garlan, N. Pecuchet, K. Perez-Toralla, C. Milbury, A. Didelot, O. Caen, H. Blons, E. Fabre, P. Laurent-Puig, V. Taly*. Plos One (2016), 11(7):e0159094.

BRAF mutation correlates with High-Risk Langerhans Cell Histiocytosis and increased resistance to first-line therapy. S. Héritier, J-F. Emile, M. Aziz-Barkaoui, C. Thomas, S. Fraitag, S. Boudjemaa, F. Renaud, A. Moreau, M. Peuchmaur, C. Chassagne-Clément, F. Dijoud, V. Rigau, D. Moshous, A. Lambilliotte, F. Mazingue, K. Kebaili, J. Miron, E. Jeziorski, G. Plat, N.  Aladjidi, A. Ferster, H. Pacquement, C. Galambrun, L. Brugières, G. Leverger, L. Mansuy, C. Paillard, A. Deville, C. Armari-Alla, A. Lutun, M. Gillibert-Yvert, J-L Stephan, F. Cohen-Aubart, J. Haroche, I. Pellier, F. Millot, B. Lescoeur, V. Gandemer, C. Bodemer, R. Lacave, Z. Hélias-Rodzewicz, V. Taly, F. Geissmann, and J. Donadieu*. Journal of Clinical Oncology, (2016), 34(25):3023-30.

High-resolution mapping of bifurcations in nonlinear DNA circuits. A.J. Genot, A. Baccouche, R. Sieskind, N. Aubert-Kato, N. Bredeche, J.F. Bartolo, V. Taly, T. Fujii, Y. Rondelez. Nature Chemistry (2016), 8(8):760-7.

A Study of Hypermethylated Circulating Tumor DNA as a Universal Colorectal Cancer Biomarker. S. Garrigou, G. Perkins, F. Garlan, C. Normand, A. Didelot, D. Le Corre, S. Peyvandi, C. Mulot, R. Niarra, P. Aucouturier, G. Chatellier, P. Nizard, K. Perez-Toralla, E. Zonta, C. Charpy, A. Pujals, C. Barau, O. Bouché, J.-F. Emile, D. Pezet, F. Bibeau, J. B. Hutchison, D. R. Link, A. Zaanan, P. Laurent-Puig, I. Sobhani and V. Taly*. Clinical Chemistry (2016), 62(8):1129-39.

2015

Quel avenir pour l’ADN tumoral circulant? Etat des lieux et perspectives dans les cancers colorectaux, pulmonaires non à petites cellules et pancréatiques. D. Pietrasz, N. Pécuchet, E. Fabre, H. Blons, V. Taly, P. Laurent-Puig, J-B Bachet*. Bulletin du cancer (2015), 103(1):55-65.

Why and how immunohistochemistry should now be used to screen for the BRAFV600E status in metastatic melanoma? The experience of a single institution (LCEP, Nice, France). Long E, Ilie M, Lassalle S, Butori C, Poissonnet G, Washetine K, Mouroux J, Lespinet V, Lacour JP, Taly V, Laurent-Puig P, Bahadoran P, Hofman V, Hofman P.* J Eur Acad Dermatol Venereol (2015) doi: 10.1111/jdv.13332. [Epub ahead of print]

Variations of BRAF mutant allele percentage in melanomas. Hélias-Rodzewicz Z, Funck-Brentano E, Baudoux L, Jung CK, Zimmermann U, Marin C, Clerici T, Le Gall C, Peschaud F, Taly V, Saiag P, Emile JF.* BMC Cancer (2015), 15:497.

Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Lim,  J., Caen, O., Vrignon, J., Konrad, M., Taly, V. and Baret, J-C.* Co-first name for Lim, J. and Caen, O. Biomicrofluidics (2015), 9(3):034101.

Assessment of DNA integrity, applications for cancer research. Zonta, E., Nizard, P. and Taly, V.* Advances in Clinical Chemistry. (2015), 70:197-246.

Digital PCR compartmentalization I. Single-molecule detection of rare mutations. Perez-Toralla, K., Pekin, D., Garlan, F., Bartolo, JF, Nizard, P., Laurent-Puig, P., Baret, JC, Taly, V.* Revue invitée (Français) pour le journal Medecine et Sciences (2015), 31(1):84-92.

Digital PCR compartmentalization II. Contribution for the quantitative detection of circulating tumor DNA. Caen, O., Nizard, P., Garrigou, S., Perez-Toralla, K. Zonta, E., Garlan, F., Laurent-Puig, P., Taly, V.* Revue invitée (Français) pour le journal Medecine et Sciences (2015), 31(2):180-6.

Microfluidics and Tumor DNA. Contribution of digital PCR (technical review in French, french title: Microfluidique et ADN tumoral. Apport de la PCR digitale). Nizard P, Krol A, Laurent-Puig P, Taly V.* Techniques de l’ingénieur (2015). In press.

Clinical relevance of KRAS-mutated sub-clones detected with picodroplet digital PCR in advanced colorectal cancer treated with anti-EGFR therapy. Laurent-Puig, P.*, Pekin, D., Normand, C., Kotsopoulos, SK, Nizard, P., Perez Toralla, K., Rowell, R., Olson, J., Srinivasan, P., Le Corre, D., Hor, T., El Harrak, Z., Li X., Link, D.R., Bouche, Emile, J-F., O., Landi, B., Boige, V., Hutchison, J.B. and Taly, V.* Clinical Cancer Research (2015), 21(5):1087-97.

2014

Recurrent RAS and PIK3CA mutations in Erdheim-Chester disease.  Emile, J-F., Diamond, E.L., Hélias-Rodzewicz, Z., Cohen-Aubart, F., Charlotte, F., Hyman, D.M., Kim, E., Rampal, R., Patel, M., Ganzel, C., Aumann, S., Faucher, G., Le Gall, C., Leroy, K., Colombat, M., Kahn, J-E., Trad, S., Nizard, P., Donadieu, J., Taly, V., Amoura, Z., Abdel-Wahab, O. and Haroch, J. Blood (2014), 124(19):3016-9.

Association of both Langerhans cell histiocytosis and Erdheim-Chester Disease linked to BRAFV600E mutation. A multicenter study of 23 cases. B Hervier, J Haroche, L Arnaud, F Charlotte, J Donadieu, A Néel, F, Lifermann, C Villabona, B Graffin, O Hermine, A Rigolet, C Roubille, E Hachulla,T Carmoi, M Bézier, V Meignin, M Conrad, L Marie, E Kostrzewa, JM Michot, S Barete, V. Taly, K Cury, JF Emile, Z Amoura. Blood (2014), 124 (7) : 1119-1126.

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics. Beneyton, T., Coldren, F., Baret, J-C., Griffiths, A.D. and Taly, V.* Analyst (2014), 139(13):3314-23. (Invited article for the themed issue on “Probe and Chip Approaches to Cell Analysis”).

The Microfluidic Puzzle: Chip-oriented Rapid Prototyping. Lim, J., Maes, F., Taly, V. and Baret, J-C. Lab on Chip (2014), 14 (10), 1669-1672.

2013

Circulating DNA, digital PCR and colorectal cancers. Taly, V and Laurent-Puig, P. Correspondances en Onco-theranostic (2013), 2(4), 184-189 (Invited review for the thematic issue : Cellules tumorales et ADN libre circulant). 

Multiplex picodroplet digital PCR to detect KRAS mutationsin circulating DNA from plasma of colorectal cancer patients. Taly, V.*, Pekin,D., Benhaim, L., Kotsopoulos, SK, Le Corre, D., Li X., Atochin, I., Link, D.R.,Griffiths, A.D., Pallier, K., Blons, H., Bouche, O., Landi, B., Hutchison, J.B.and Laurent-Puig, P. Clinical Chemistry (2013), 59(5):815-823.

High-throughput formation and control of monodisperse liquidcrystals droplets driven by an AC fields in a microfluidic device. Belloul, M.,Bartolo, J-F., Ziraoui, B., Coldren, F., Taly, V., El Abed, A.I.* AppliedPhysics Letters (2013), 103 (3), 033112. http://dx.doi.org/10.1063/1.4813880

Real-time detection of Whispering Gallery Mode resonance inhigh-throughput flowing monodisperse microdroplets. El Abed, A.I.* and Taly, V. Optical Materials (2013). 36(1): Pages 64–68.

Multiplex picoliter-droplet digital PCR for quantitativeassessment of DNA integrity in clinical samples. Didelot A, Kotsopoulos SK,Lupo A, Pekin D, Li X, Atochin I, Srinivasan P, Zhong Q, Olson J, Link DR,Laurent-Puig P, Blons H, Hutchison JB, Taly V. Clinical Chemistry (2013),59(5):815-823.

Membraneless Glucose/O2 microfluidic biofuel cells usingcovalently bound enzymes. Beneyton, T., Putu Mahendra Wijaya, I.  , C. Ben Salem, C., Griffiths, A.D. andTaly, V. Chemical communications (2013), 49(11): 1094-1096.

2012

Dynamics of molecular transport by surfactants in emulsions.Skhiri, Y., Gruner, P., Semin, B., Brosseau, Q., Pekin, D., Mazutis, L., Goust,V., Kleinschmidt, F., El Harrak, A., Hutchison, J. B., Mayot, E., Bartolo,J-F., Griffiths, A.D. , Taly, V.* and Baret, J-C.*. Soft Matter (2012), 8:10618-10627. Journal Cover. 

Detecting Biomarkers with microdroplet technology. Taly,V.*, Pekin, D, El Abed A. and Laurent-Puig, P. Invited review. Trends inMolecular Medicine. 2012 Jul;18(7):405-16. Journal cover.

2011 

Sensitive detection of rare mutations using droplet-basedmicrofluidics. Baret J.-C. and Taly V. G.I.T. Journal Laboratory Europe (2011),15: 12-13. 

Quantitative and sensitive detection of rare mutations usingdroplet-based microfluidics. Pekin, D., Skhiri, Y., Baret, J.-C., Le Corre, D.,Mazutis, L., Ben Salem, C., Millot, F., El Harrak, A., Hutchison, J.B., Larson,J.W., Link, D.R., Laurent-Puig, P., Griffiths, A.D., and Taly, V.* Lab on achip (2011), 11(13): 2156-66. Journal inside cover Picture. 

Highlights in: www.genomeweb.com

Press Release by CNRS, Université de Strasbourg, UniversitéParis-Descartes and Max Plank Institute (seehttp://www2.cnrs.fr/presse/communique/2187.htm and followed by Articles inFrench Press) as well as in the CNRS international Journal. Television (5) andradio (2) interviews.

Multiplex Digital PCR: Breaking the One Target Per Color Barrier of Quantitative PCR. Zhong,Q., Bhattacharya, S., Kotsopoulos, S., Olson, J., Taly, V., Griffiths, A.D.,Link, D.R., and Larson. J.W., Lab on a chip (2011), 11(13):2167-74.

Immobilisation of CotA, an extremophilic laccase fromBacillus subtilis, on glassy carbon electrodes for biofuel cell applications.Beneyton, T., El Harrak, A., Griffiths, A.D. and Taly, V.* ElectrochemistryCommunications (2011), 13(1): 24-27.

KRAS mutation detection trap. Benhaim, L., Maley, K., LeCorre, D., Blons, H., Taly, V., Bibeau, F., Emile, J-F. and Laurent-Puig, P.Journal of Clinical Oncology (2011), Letter, 29(8): e208-e209.

From Toxins Targeting Ligand Gated Ion Channels toTherapeutic Molecules. Nasiripourdori, A., Taly, V., Grutter, T. and Taly, A.Toxins (2011), 3(3): 260-293.

The thermophilic CotA laccase from Bacillus subtilis:Bioelectrocatalytic evaluation of O2 reduction in the direct and mediatedelectron transfer regime. Beneyton, T., Beyl, Y., Guschin, D., Griffiths, A.D.,Taly, V. and Schuhmann, W. Electroanalysis (2011), 23 (8): 1781-9.

2010 

Tiny droplets for high-throughput cell-based assays. Baret,J.-C. and Taly, V. In Unravelling more of cell genomics : let’s use the microand nanotools !, RCS publication edited by M.-C. Potier and N. Bontoux. (2010)261-84.

2009

Microfluidic fluorescent activated droplet sorting (mFADS)for biological applications. Baret, J.-C., Miller, O.J., Taly, V., El Harrak,A., Ryckelynk, M., Agresti, J., Weitz, D. and Griffiths A.D. Co-first name forBaret JC and Miller OJ. Lab on a Chip (2009), 9(13): 1850-8. Journal coverPicture.

Highlights in: "Highlights in Chemical Biology", rsc publishing. Cell sortingsorted (2009)

Droplets and Emulsions: very high-throughput in biology.Baret, J.-C., Taly, V., Ryckelynk, M. and Griffiths A.D. Medecine et sciences(2009), 25(6-7): 6. 

Droplet-based microfluidic systems for high throughputsingle DNA molecule amplification and analysis. Mazutis L., Fallah-Araghi, A.,Miller, O. J., Baret, J. C., Frenz, L., Janoshazi, A., Taly, V., Miller, B. J.,Hutchison, B. J., Link, D., Griffiths, A.D. and Ryckelynck, M. AnalyticalChemistry (2009), 81(12): 4813-21. 

Multi-step microfluidic droplet processing: kinetic analysisof an in vitro translated enzyme. Mazutis L., Baret, J. C., Treacy, P., Skhiri,Y., Fallah-Araghi, A., Ryckelynck, M., Taly, V.* and Griffiths, A.D.*  Lab on a Chip (2009), 9: 2902-8.

2007

Miniaturizing chemistry and biology in microdroplets. Kelly,B.T., Baret, J.C., Taly, V. and Griffiths, A.D. Chemical Communications(Cambridge, UK) (2007), 18: 1773-88.

A combinatorial approach to substrate discrimination in theP450 CYP1A subfamily. Taly, V., Urban, P., Truan, G. and Pompon, D. Biochim.Biophys. Acta. (2007), 1770(3): 446-57.

Droplets as microreactors for High-Throughput Biology. Taly,V., Kelly, B.T. and Griffiths, A.D. ChemBioChem. (2007), 8(3):263-72.

2006

Directed evolution by in vitro compartmentalization. Miller,O.J., Bernath, K., Agresti, J.J., Amitai, G., Kelly, B.T., Mastrobattista, E.,Taly, V., Magdassi, S., Tawfik, D.S. and Griffiths, A.D. Nature Methods (2006),3(7): 561.

2005

High-throughput screening of enzyme libraries: in vitroevolution of a beta-galactosidase by fluorescence-activated sorting of doubleemulsions. Taly, V., Mastrobattista, E., Chanudet, E., Treacy, P., Kelly, B.and Griffiths, A. Co-first name. Chemistry and Biology (2005), 12(12):1291-300.

Highlights in:

- Nature. Vol. 440. 2006. Cornish V.W. New and views.Catalytic competition for cells. pp.156-157.

- Nature Methods. Vol. 3(2). 2006. Tiny droplets make a bigsplash. Eisenstein M. pp.71.

- Chemistry and Biology. Vol. 12, 1255-1258, 2005. Previews.O'Hare H.M. and Jonhsson K. The laboratory in a Droplet.

2003

Microarray based method for combinatorial library sequencemapping and characterization. Abecassis V., Jaffrelo L., Rickman D., AggerbeckL., Herbert C., Truan G. and Pompon D. Biotechniques (2003), 34: 1272-1279. 

Sequence mapping of combinatorial libraries on macro- andmicro-arrays: experimental design of DNA arrays. Abecassis, V., Truan, G.,Jaffrelo, L., and Pompon, D. In Methods in Molecular Biology (2003), 231:189-198. Directed Evolution Library Creation: Methods and Protocols. HumanaPress edited by F. H. Arnold.

Producing chimeric genes with the CLERY method: use of invitro and in vivo recombination techniques. Abecassis, V., Pompon, D., andTruan, G. In Methods in Molecular Biology (2003), 231: 165-173. DirectedEvolution Library Creation: Methods and Protocols. Humana Press edited by F. H.Arnold.  

Sequence mapping of combinatorial libraries on macro- andmicroarrays: bioinformatic treatement of data. Pompon, D., Truan, G. andAbecassis, V. In Methods in Molecular Biology (2003), 231: 199-211. DirectedEvolution Library Creation: Methods and Protocols. Humana Press edited by F. H.Arnold. 

Computational methods for sequence mapping of largecombinatorial libraries and deduced sequence signatures. Abecassis V.,Aggerbeck L., Truan G. and Pompon D. Biotechniques (2003), 34: 1280-1286.

Exploration of natural and artificial sequence spaces:towards a functionnal remodelling of membrane-bound cytochrome P450s. AbecassisV., Urban P., Truan G. and Pompon D. Biocatalysis and Biotransformation (2003),21 (2): 55-66.

2001

Design and characterization of a novel “family-shuffling”technology adapted to membrane enzyme: application to P450s involved inxenobiotic metabolism. Abecassis, V., Pompon, D. and Truan, G. Advance inExperimental Medicine and Biology (2001), 500: 319-322.

2000

High efficiency family shuffling based on multi-step PCR andin vivo DNA recombination in yeast: statistical and functional analysis of acombinatorial library between human cytochrome P450 1A1 and 1A2. Abecassis V.,Pompon D. and Truan G. Nucleic Acid Research (2000), 28: e88

PATENTS

Fluorocarbon emulsion stabilizing surfactants. Holtze, C.,Guerra, R.E., Agresti, J., Weitz, D., El Harrak, A., Miller, O., Baret, J.C.,Taly, V., Merten, C., Ryckelynck, M., Griffiths, A.D. WO/2008/021123.

Compositions and methods for in vitro sorting of molecularand cellular libraries. Tawfik, D., Bernath, K. , Aharoni, A., Peisajovich, S.,Griffiths, A.D., Mastrobattista, E., Magdassis, S., Taly, V., Chanudet, E.WO/2005/049787.

Improved combinatorial libraries by recombination in yeastand analysis method. Abecassis, V., Pompon, D. and Truan, G. CNRS/AventisPharma. WO/2001/096555

PROCEEDING ARTICLES (peer-reviewed)

Prognostic value of circulating tumor DNA in advanced colorectal cancer patients: quantification of hypermethylated or mutant sequences using picoliter-droplet digital PCR. F. Garlan, P. Laurent-Puig, N. Siauve, A. Didelot, G. Perkins, H. Blons, J. Taieb, V. Taly, A. Zaanan. Proceedings: AACR 107th Annual Meeting 2015, Avril 16-20, 2016; New Orleans, US.

Recurent RAS and PIK3CA mutations in Erdheim-Chester disease and histiocytic sarcoma. J. Haroche, E.L. Diamond, J-F Emile, Z. Helias-Rodzewicz, F. Cohen-Aubart, R. Charlotte, D.M. Hyman, R. Rampal, M. Patel, C. Ganzel, S. Aumann, G. Faucher, C. Le Gall, K. Leroy, M. Colombat, J.E. Kahn, S. Trad, P. Nizard, P J. Donadieu, V. Taly, Z. Amoura, O. Abdel-Wahab. Pediatric Blood & cancer, 62(1), p. S9-S10

Circulating tumor DNA as a prognostic marker in colorectal cancer: Preliminary results of a prospective trial. Pierre Laurent-Puig, Olivier Bouché, Ralph Niarra, Pascaline Aucouturier, Leonor Benhaim, Bruno Landi, Anne Berger, Thierry Lecomte, Corinne Normand, Delphine Le Corre, Audrey Didelot, Karine Mallet, Karla Perez Toralla, Thevy Hor, Zakaria El Harrak, Gilles Chatellier, Brian Hutchison, Darren Link, Valerie Taly. Proceedings: AACR 106th Annual Meeting 2015, Avril 18-22, 2015; Philadelphia, US.

Assessing cancer drug resistance at the single-cell level. O. Caen, E. Zonta, H. Lu, J. Vrignon , P. Nizard, J-C. Baret and V. Taly The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. Novembre 2014.

High-resolution landscape of a molecular program. A.J. Genot, A. Baccouche, R. Sieskind, N. Aubert, N. Bredeche, J.F. Bartolo, V. Taly, T. Fujii, Y. Rondelez. Extended proceedings of DNA20 conference (2014) Kyoto, Japan.

Picoliter droplet-based digital PCR to quantify circulating DNA in plasma of metastatic colorectal cancer patients. Garlan F., Didelot A., Perkins G., Rice N., Zaanan A., Laurent-Puig P. and Taly V. The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. November 2014.  

Pipette-and-play: parallelized ultra-high throughput microfluidic emulsifier for quantitative biochemical assays. J. Lim, O. Caen, J. Vrignon, M. Konrad, V. Taly and J.C. Baret. In: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), p. 258-260. ISBN: 978-0-9798064-7-6.

Clinical significance of low frequency KRAS and BRAF subclones for advanced colon cancer management. Valerie Taly, Pierre Laurent-Puig, Deniz Pekin, Corinne Normand, Steve K. Kotsopoulos, Philippe Nizard, Karla Perez-Toralla, Rachel Rowell, Jeff Olson, Preethi Srinivasan, Delphine Le Corre, Thevy Hor, Zakaria El Harrak, Xinyu Li, Qun Zhong, Darren R. Link, Olivier Bouché, Jean-François Emile, Bruno Landi, Valérie Boige, J. Brian Hutchison. Proceedings: AACR 105th Annual Meeting 2014; Apr 5-9, 2014; San Diego. Published in Cancer Research October 1, 2014 ; 74:2820; DOI:10.1158/1538-7445.AM2014-2820. 

Quantitative cancer analysis using digital PCR: Absolute counting of DNA (solid tumors and liquid biopsies in glioma, breast, and colon cancer) and RNA (mRNA and miRNA using One-Step RT-dPCR). Michael L. Samuels, Valerie Taly, Leonora Balaj, Saumya Das, and Ben H. Park. Proceedings: AACR 105th Annual Meeting 2014, Avril 5-9, 2014; San Diego, CA. Published in Cancer Research, October 1, 2014 74:5316; DOI: 10.1158/1538-7445.AM2014-5316.

Peptide Nucleic Acid Fluorescent Probes For Detection Of Specific Biomarkers for digital microfluidics systems. Jean-Francois Bartolo, Sylain Ladame and Valerie Taly. The International Conference for Micro& Nanotechnologies for the Biosciences, NanoBioTech-Montreux, Switzerland. Novembre 2013.  

Detection and quantification of minoritary subclones of KRAS in metastatic colorectal cancers by digital microfluidics: therapeutic implications. D. Pekin, C. Normand, S. Kotsopoulos, X. Li, L. Benhaim, O. Bouché, T. Lecompte, D. Le Corre, T. Hor, Z. El Harrak, P. Nizard, D. Link, J.B. Hutschinson, P. Laurent-Puig and V. Taly. Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. Published in Cancer Research Avril 15, 2013; 73(8 Supplement): 4211. DOI:10.1158/1538-7445.AM2013-4211

Microfluidic Approaches for the Study of Emulsions: Transport of Solutes. Philipp Gruner, Yousr Skhiri, Benoit Semin, Quentin Brosseau, Andrew D Griffiths, Valérie Taly, Jean-Christophe Baret. MRS Proceedings / Volume 1530 / 2013, DOI: http://dx.doi.org/10.1557/opl.2013.80.

Picoliter droplet-based digital PCR for the quantitative andsensitive detection of rare mutations and the assessment of DNA integrity. Deniz Pekin, Audrey Didelot, Steve K. Kotsopoulos, Jean-Christophe Baret,Delphine Le Corre, Brian Hutchison, Darren R. Link, Pierre Laurent-Puig, AndrewD. Griffiths, and Valérie Taly. Proceedings of the 3rd European Conference onMicrofluidics - Microfluidics 2012 - Heidelberg, December 3-5, 2012

Quantitative detection of circulating tumor DNA bydroplet-based digital PCR. Valerie Taly, Deniz Pekin, Steve Kotsopoulos, XinyuLi, Ivan Atochin, Hu Gang, Delphine Le Corre, Leonor Benhaim, J. BrianHutchison, Darren R. Link, Hélène Blons, Pierre Laurent-Puig. AmericanAssociation for Cancer annual meeting (AACR 2012). March 31-April  4th, Chicago, USA.

Quantitative detection of circulating tumor DNA in plasmasamples by droplet digital PCR. Pekin D., Kotsopoulos S., Xinyu L.,Atochin I., Gang H., Le Corre D., Benhaim L., Hutchison J. B., Link D.R., Blons H., Laurent-Puig P. and Taly V.,* The 16th Internationalconference on miniaturized systems for Chemistry and Life Science (MicroTas2012). Oct. 28- Nov. 1, 2012. Okinawa. Japan.

Multiplex highly sensitive detection of cancer biomarkers inbiological samples. D. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis,L., Ben Salem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. In: Proceedings of the 15th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2011, p. 665-667. ISBN: 978-0-9798064-4-5.

Integrated microfluidic platform for directed evolution oflaccase for Biofuel cell applications. Skhiri, Y., Beneyton, T., El Harrak, A.,Griffiths, A.D. and Taly, V. In: Proceedings of the 14th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2010, p. 980-982. ISBN:978-0-9798064-3-8.

Droplet-based microfluidics for the quantitative detectionof rare mutations. D. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis,L., Ben Salem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. In: Proceedings of the 14th InternationalConference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS),2010, p. 58-60. ISBN:978-0-9798064-3-8.

Mining the functional sequence space of shuffled P450s.Urban, P., Truan, G., Abecassis, V., and Pompon, D. 7th European ISSX Meeting,2004, Published in Drug Metabolism Reviews, 36 (1) : 253.

Yeast as a versatile tool for functional expression,characterization and engineering of P450s and associated redox enzymes. Pompon,D., Abecassis, V., Truan, G., Urban, P. May 21.–26th 2002. FEBS course:“Cytochrome P450 systems: from structure to application”. Kranjska gora,Slovenija 

INVITED CONFERENCES AND SEMINARS

Clinical validation of droplet-based procedures in the context of liquid biopsies (Lecture). EMBL Course- Liquid Biopsy, 9 - 13 Oct 201, Heidelberg, Germany 

Circulating tumor DNA for cancer patient follow up. Personalized Cancer Treatment meeting, Bergen 2017, 28-29 Sept. 2017. Bergen, Norway

New strategies for the highly sensitive detection of cell free circulating tumor DNA : Applications to cancer patient’ follow up. Dreyfus symposium, 21-22 sept. 2017, Paris, FR,

Droplet-based microfluidics for cancer patient follow-up: Tracking ctDNA in liquid biopsies. Precision Cancer Medicine: Forefront Technologies at the Clinical Interface, IFLS conferences, Southampton, UK, Sept. 2017

Quantitative detection of circulating tumor DNA for the follow up of cancer patients. Invited seminar, 13th June 2017, Aarhus university, Danemark

Droplet-based digital PCR for cancer research: Application to liquid biopsies. Porto Cancer Meeting, 11-12 May 2017, Porto, Portugal

Droplet-based microfluidics for single cell and single molecule analysis. Statistical Challenges in Single-Cell Biology workshop, April 30 to May 5, 2017, Ascona, Switzerland

Droplet based digital PCR for cancer patient follow-up. qPCR dPCR & NGS 2017 event, 3 - 7 April 2017, Freising, Germany

Droplet-based microfluidics: towards a new tool for cancer research. ELRIG’s Research and Innovation (R&I) Conference, March 29-30 2017,Cambridge, UK

Digital PCR and circulating plasmatic DNA: a multi-cancers analysis. Annual meeting for Grant awardees of Merieux Institute. Les Pensières conference center, October 2016, Annecy, FR.

Digital PCR for cancer patient follow up. Diagnostics Summit 2016 - Circulating Biomarkers for Liquid Biopsy Development and Companion Diagnostics, 13th-14th October 2016, Singapore. 

Droplet based microfluidics for single cell analysis. 4th Annual Single Cell Analysis Congress 2016, 10 -11 November 2016, London, UK.

From droplet-based microfluidics to digital PCR : The development of a new tool for cancer patient follow-up. NanoBio Surfaces and Interfaces in Healthcare and Science Event, 27-28 September 2016, Twente, NL.

Droplet based digital PCR for cancer patient follow up. Precision medicine based on liquid biopsies "from detection to dissection" symposium, 15 -16 September 2016, Granada, Spain.

Droplet digital PCR, liquid biopsies and patient follow-up. qPCR and Digital PCR Congress USA, Philadelphia, USA, 11-12 July 2016.

Microfluidics for cancer research. IC-ANMBES 2016, Brasov, Romania, June 2016.

Circulating tumor DNA as a new tool to follow cancer patients. Ecole thématique Hétérogénéité des tumeurs, Provins, France, May 2016.

Microfluidics for cancer research: a new tool for patients’ follow up. Journées de la federation CICB, Université Paris Descartes, France, May 2016.

Using Droplets to Highlight and Follow Cancer Genetic Markers. Molecular Dx Europe meeting, Lisbon, April 2016.

Microfluidics for cancer research: a new tool for patients’ follow up. Journée commune UFR Maths informatique et UFR biomédicale, Univ. Paris Descartes, January 2016.

Détecter et quantifier l’ADN tumoral circulant pour le suivi des patients atteints de cancers. Journées Onco-pharmacologie, Institut Cochin, Paris, France, 4th December 2015.

Tracking circulating tumor DNA using Picoliter droplet-based digital PCR. dPCR conference, San Diego, US, 2-3 November 2015.

Picoliter droplet-based microfluidics for cancer research: from the detection of low frequency KRAS subclones within tumors to the analysis of liquid biopsies. Microfluidics, London, UK, 20-21 October 2015.

Picoliter droplet-based digital PCR for cancer patient follow-up. Biotechnica 2015, Personalized medicine and its impact in the Clinic, Hanover, Germany, 7-8 Octobrer 2015.

Droplet based microfluidics to follow cancer genetic marker in the blood. NanoBlood conference, Paris, France, 6 July 2015

Droplet based microfluidics for cancer research : from the detection of low frequency KRAS subclones within tumors to the analysis of liquid biopsies. Invited conference, Institut du cerveau et de la moelle épinière (ICM), Paris, France, 3 July 2015

Monitoring circulating tumor DNA using picoliter droplet based digital PCR. qPCR and digital PCR congress USA, San Diego, 25-26 June 2015

Picoliter Droplet-Based Digital PCR for the Quantitative Analysis of Circulating Tumor DNA for Cancer Patient Follow Up. Festival of genomics, Raindance Technologies Workshop, Boston, US, 22 June 2015

PCR en microcompartiments : intérêt pour la recherche clinique. Colloque DIM analytics " La miniaturisation des mesures : quels espoirs, quels challenges ?", Paris, France, 17 April 2015

Picoliter Droplet-Based Digital PCR for the Quantitative Analysis of Circulating Tumor DNA for Cancer Patient Follow Up. Molecular Diagnostics Europ conference, Lisbon, Portugal, 15-16 April 2015

Picoliter Droplet-Based Digital PCR and microRNA Quantification. Extracellular Markers summit, Cambridge, US, 19-20 March 2015

Picoliter droplet digital PCR for cancer patient follow-up : application to colorectal cancer. 2nd qPCR & Digital PCR Congress, London, UK, 20-21 October 2014

Picoliter droplet digital PCR for cancer research. Taly V. Invited conference. Biopathology symposium. Paris, France, 19-20 June 2014.

Tiny droplets to detect cancer biomarkers. Taly V. Invited conference. 5th International Conference on Biomarkers and Clinical Research.  University of Oxford, St Hilda's College - UK, April 15-17, 2014.

Droplet digital PCR as a new tool for cancer research. Taly V. Invited conference. Advances in qPCR & dPCR conference .  Barcelona, Spain. 14-15 May 2014. 

Picoliter Droplet-Based Digital PCR for Molecular Diagnostics. Taly V. Invited conference. Molecular Medicine Tri conference 2014. San francisco, USA. 9-14 February 2014.

On the Journey to Personalised Follow ups: Using Circulating Tumour DNA in Colorectal Cancer Patients. Taly V. Invited conference. Circulate Europe - Berlin, Germany. 18-20 february 2014. Additional authors: Aziz Zaanan, Hélène Blons, Audrey Didelot, Géraldine Perkins, Nadejda Rice, Fanny Garlan, Philippe Nizard, Julien TAIEB and P. Laurent-Puig.

L’apport de la microfluidique à la PCR numérique dans la détection des biomarqueurs. Taly V. Invited conference. Journées Internationales de Biologie-  Paris, France. 13-15 November 2013.

How Digital PCR Simplifies Screening for Multiple Cancer Biomarkers. Taly, V. Invited Raindance Digital PCR webinars, 16th October 2013.

Droplet-based microfluidics as a tool for personalizedmedicine. Taly V. Invited conference. Digital PCR conference. San Diego, US, October 6-9 2013.

Detection of low frequency alleles in patient samples bydigital PCR. Taly V. Invited conference. qPCR and dPCR congress. Lyon, France,September 2013.

Digital PCR strategies in the development and analysis ofmolecular biomarkers for personalized medicine. Taly V. Invited conference (onbehalf of Prof. P. Laurent-Puig). qPCR and dPCR congress. Lyon, France,September 2013.

Droplet-based microfluidics for cancer biomarker screening.Taly, V. Invited Conference. Societé de pharmaco-toxicologie cellulaire (SPTC)annual conference. Chatenay-Malabry. France. 6th June 2013.

Droplet-based microfluidic for cancer research: Applications for personalized medicine. Taly, V. Invited Seminar. Microfluidique, MEMS,Nanostructures (MMN group), Gulliver Lab, ESPCI. Paris. France. May 21 2013.

Droplet-based microfluidic for cancer research: Applicationsfor personalized medicine. Invited Seminar. Microfabrication and cell biologyclub. May 3rd 2013.

Microdroplets for High-throughput screening of biologicaland chemical reactions : towards the development of new procedures for cancerresearch. IMTCE Seminar. Paris. January 2013.

Detection of cancer biomarkers in microdroplets. Taly, V.Invited Speaker. The International Conference for Micro& Nanotechnologiesfor the Biosciences, NanoBioTech-Montreux, Switzerland. November 12-142012. 

Microfluidics, droplets and Cancer research. Taly, V.Invited Speaker. Kagawa University Mini-conference on Novel devices applicableto bio/clinical/medical fields, Takamatsu, Japan. November 2, 2012.

Droplet-based Microfluidics for the Highly SensitiveDetection of Cancer Biomarkers in Biological Samples. Taly, V. Invited Speaker.Euromediag International Convention, 5th July 2012. Montpellier. France.

Tiny droplets to fight cancer. Taly, V. Invited Seminar.Laboratory for Photonics and Nanostructures (LPN), 5th June 2012. Paris.France.

Droplet-based Microfluidics for the High SensitivityDetection of Cancer Biomarkers in Biological Samples. Taly, V. Invited Speaker.Nano and Microfluidics conference. 30-31 May 2012. Hamburg. Germany.

Tiny droplets to fight cancer. Taly, V. Invited Seminar.Institut Jacques Monod, Université Paris 7. 20th March 2012. Paris. France.

Droplet-based microfluidics for biological applications:From enzymatic screening to biomarker analysis. Taly, V. Invited Seminar.Twente University. 6 February 2012. Enschede. Netherland.

Multiplex highly sensitive detection of Cancer Biomarkers inbiological samples. D. Pekin, S. Kotsopoulos, H. Gang, Q. Zhong, J-C. Baret, D.Le Corre, A. El Abed, J. B. Hutchison, D. Link, J. Larson, A. Griffiths, P.Laurent-Puig and V. Taly. Invited by Raindance Technologies. Association ofMolecular Pathology (AMP) 2011 Annual meeting workshop. 19 November 2011,Dallas, US.

How microdroplets could help fighting Cancer. Invited Speaker.Scientific days of the Ecole Doctorale Biologie-Santé Nantes (France). 8November 2011. Sable d’Olonne. France.

Droplet-based microfluidic for the quantitative detection ofrare mutations. Pekin, Y. Skhiri, Baret, J.-C., Le Corre, D., Mazutis, L., BenSalem, C., El Abed, A., Hutchison, J.B., Link, D.R., Griffiths, A.D.,Laurent-Puig, P. and Taly, V. Invited Speaker. MicroTas Conference. 4-7 October2010. Groningen. Netherlands.

Microfluidics. Invited seminar, UMRS-775 (Paris Descartesuniversity). 14 June 2010. Etampes. France.

Tiny droplets for single-cell analysis: From enzymaticscreening to cell sorting. Lorentz workshop "micro- and nanofluidics forcell biology". Invited Speaker. 17-22 January 2010.

Minaturizing the Laboratory in Emulsion Droplets. Stepsevent 2009, Meeting for french, german and swiss scientists in life sciences(funding companies/researchers) organised by the Alsace BioValley cluster. 15thDecember 2009. Strasbourg. France. 

Tiny droplets for cell-based assays. 2nd German / French /European Meeting on Yeast and Filamentous Fungi. 28-30 May 2009. Strasbourg.France.

Microfluidic systems for High-throughput screening anddirected evolution. Conectus meetings between Alsacian Companies and ResearchLaboratories. 16th October 2008.

Miniaturizing laboratory in emulsion droplets: applicationto toxicology. Invited seminar in the laboratory of Pr. P. Laurent-Puig, INSERMUMR S775, University Paris Descartes, June 2008, Paris, France.

Miniaturizing the Laboratory in Emulsion Droplets. Taly V.and Griffiths A. Next generation Therapeutics. November 2006, Basel,Switzerland.

Directed evolution of enzymes using selection ofdouble-emulsions by FACS. Application to the design of biocatalysts for biofuelcell. Taly V. and Griffiths AD. Invited seminar in the laboratory of Pr. H.Goerish, November 2004, Technical University of Berlin, Germany.

OTHER ORAL COMMUNICATIONS (selection).

Droplet-based microfluidic for cancer research: Applicationsfor personalized medicine. Taly, V. Invited by Raindance Technologies. qPCR andNGS 2013 event. Freising, Germany. 18-22 march 2013.

Quantitative detection of circulating tumor DNA in plasmasamples by droplet digital PCR. Pekin D., Kotsopoulos S., Xinyu L., Atochin I.,Gang H., Le Corre D., Benhaim L., Hutchison J. B., Link D. R., Blons H.,Laurent-Puig P. and Taly V.* The 16th International conference on miniaturizedsystems for Chemistry and Life Science (MicroTas 2012). Oct. 28- Nov. 1, 2012.Okinawa. Japan.

Emulsion PCR in microfluidics for the quantitative detectionof rare mutations: Towards an application in cancer diagnostics. D. Pekin, Y.Skhiri, J-C. Baret, D. Le Corre, L. Mazutis, C. Ben Salem, A. El Abed, J. B.Hutchison, D. Link, J. Larson, A. Griffiths, P. Laurent-Puig and V. Taly. GDR2825- New Approaches in Protein Directed Evolution. October 11-12th 2011.Ax-les-thermes, France. 

Multiplex highly sensitive detection of Cancer Biomarkers inbiological Samples. D. Pekin, Y. Skhiri, J-C. Baret, D. Le Corre, L. Mazutis,C. Ben Salem, A. El Abed, J. B. Hutchison, D. Link, J. Larson, A. Griffiths, P.Laurent-Puig and V. Taly. MicroTas Conference. 2-6 October 2011. Seattle. USA.

Quantitative detection of rare mutations using droplet-basedmicrofluidics. Taly V. Interbio Scientific Workshop. Microfluidics, from singlemolecule to cell biology. June 29th 2011. Institut Européen de Chimie etBiologie. Bordeaux, France.

Emulsion PCR in microfluidics for the quantitative detectionof rare mutations: Towards an application in cancer diagnostics. D. Pekin, Y.Skhiri, J-C. Baret, D. Le Corre, L. Mazutis, C. Ben Salem, A. El Abed, J. B.Hutchison, D. Link, J. Larson, A. Griffiths, P. Laurent-Puig and V. Taly.Micro- and Nano-fluidic GDR. May 23-25th 2011. Lyon, France.

Communications within the CNRS "research Group" «Protein Directed evolution» (GDR EDP, 2825). 1-2 meetings per year since 2005(oral presentations).

Design and characterization of a novel “family-shuffling”technology adapted to membrane enzyme: application to P450s involved inxenobiotic metabolism. Abecassis V., Pompon D. and Truan G. July 2000. 6thInternational Symposium on Biological Reactive Intermediates. Paris, France.

New DNA-shuffling approaches adapted to membrane enzymes:Library analysis and mosaic structure characterization. Abecassis V., QuineyC., Pompon D. and Truan G. September 2001. International conference of P450. LaGrande-Motte, France.

Screening P450 library: Living cell, microsomal preparationor permeabilized cell? Abecassis V., Nahon P., Pompon D. and Truan G. Septembre2001. Workshop Cytochromes P450 in bioréactors. International conference ofP450. La Grande-Motte, France. 

Functional analysis of mosaic P450 generated byDNA-shuffling strategy. Abecassis V., Quiney C., Jaffrelo L., Simoes N., TruanG. and Pompon D. May 21. – 26th 2002. FEBS course: “Cytochrome P450 systems:from structure to application”. Kranjska gora, Slovenia. 

Directed evolution of enzymes using selection ofdouble-emulsions by FACS. Taly V., Mastrobattista E., Chanudet E., KhodaverdiV. and Griffiths A. October 2004. Meeting of the ENDIRPRO Research TrainingNetwork (5th Framework). Louvain-la-neuve, Belgium.