Scientists involved: Ouriel Caen, Hélène Guermouche, Yoann Leveque, Heng Lu, Mani Sai Suryateja Jammalamadaka, Roberta Menezes, Dr Philippe Nizard, Georgios Pavlou, Dr Karla Perez-Toralla 

Scientists involved previously: Jean-François Bartolo, Zakaria El Harrak, Deniz Pekin, Yousr Skhiri, Beneyton Thomas, Linas Mazutis.

We aim at creating new generations of devices, tools and procedures for biological and chemical applications. Our research is dedicated to develop and use microfluidic system to perform experiments that are out of the possibilities of existing technologies. Within other, our projects aim at : (i) developping microfluidic platforms and strategies allowing to characterize and understand tumor heterogeneity and its implication for cancer research and treatment management; (ii) creating new generations of microfluidic devices for the non-invasive detection of cancer biomarkers with applications in personalized medicine, cancer recurrence detection and cancer diagnostics.

COLLABORATORS

Dr Jean-Christophe Baret. Droplets, Membranes & Interfaces Group. Max Planck Institute for Dynamics and Self-organization. Goettingen, Germany & Centre de Recherche Paul Pascal (CRPP), Bordeaux, France.

Prof. Andrew Griffiths. Laboratory of Chemical Biology. ESPCI. Paris. France. 

Dr Sylvain Ladame. Biosensor Development Group. Imperial College. London, UK.

Dr Yannick Rondelez. LIMMS/CNRS (university of Tokyo, Japan) and ESPCI, Paris, FR.

Raindance Technologies. Boston. USA.

Fluigent. Paris. France.

FUNDINGS

Ligue Nationale contre le cancer (Labelization), CARPEM (Cancer research for personalized medicine SIRIC network), Merieux Institute (Advanced Research Grant, PLP & VT), French National Alliance for Life Sciences and Health (Aviesan, PhD fellowships), INSERM (Physics, Mathematics and Engineering sciences applied to the Cancer Research, Physicancer program)

INSTITUTIONAL SUPPORT

CNRS, INSERM, Université Paris-Descartes

PUBLICATIONS

High-resolution mapping of bifurcations in nonlinear DNA circuits. A.J. Genot, A. Baccouche, R. Sieskind, N. Aubert-Kato, N. Bredeche, J.F. Bartolo, V. Taly, T. Fujii, Y. Rondelez. Nature Chemistry (2016), 8(8):760-7. Pubmed.

Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Lim,  J., Caen, O., Vrignon, J., Konrad, M., Taly, V. and Baret, J-C.* Co-first name for Lim, J. and Caen, O. Biomicrofluidics (2015), 9(3):034101. Pubmed.

Microfluidics and Tumor DNA. Contribution of digital PCR (technical review in French, french title: Microfluidique et ADN tumoral. Apport de la PCR digitale). Nizard, P., Krol, A., Laurent-Puig, P. and Taly, V. Techniques de l'ingénieur (2015). In press.

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics. Beneyton, T., Coldren, F., Baret, J-C., Griffiths, A.D. and Taly, V.* Analyst (2014), 139(13):3314-23. (Invited article for the themed issue on “Probe and Chip Approaches to Cell Analysis”). Pubmed.

The Microfluidic Puzzle: Chip-oriented Rapid Prototyping. Lim, J., Maes, F., Taly, V. and Baret, J-C. Lab on Chip (2014), 14 (10), 1669-1672. Pubmed.

High-throughput formation and control of monodisperse liquid crystals droplets driven by an AC electric field in a microfluidic device. Belloul, M., Bartolo, J-F., Ziraoui, B., Coldren, F., Taly, V. and El Abed, A.* Applied  Physics Letters (2013). In press.

Real-time detection of Whispering Gallery Mode resonance in high-throughput flowing monodisperse microdroplets. El Abed, A.I.* and Taly, V. Optical Materials (2013). In press.

Dynamics of molecular transport by surfactants in emulsions. Skhiri, Y., Gruner, P., Semin, B., Brosseau, Q., Pekin, D., Mazutis, L., Goust, V., Kleinschmidt, F., El Harrak, A., Hutchison, J. B., Mayot, E., Bartolo, J-F., Griffiths, A.D. , Taly, V.* and Baret, J-C.*. Soft Matter (2012), 8: 10618-10627. Journal Link.